COLORING MATTER
Pure mustard is a very light dull yellow, and whenever the sample is bright yellow, there is good grounds for suspecting the presence of some artificial coloring matter.
Turmeric
Add strong ammonium hydroxid to the mustard, and if turmeric is present an orange-red color is usually produced.
Make an alcoholic extract of the sample and dip a piece of filter paper in it, and when dry draw it through a cold, saturated solution of boric acid in water. An orange or red-brown tint produced on the paper indicates the presence of turmeric.
Thoroughly mix 2 or 3 grams of the mustard with castor oil and filter. If turmeric is present the filtrate will appear fluorescent.
Extract a portion of the sample with 3 times its weight of wood alcohol and filter. Evaporate one half of the solution to dryness and add a little hydrochloric acid to the residue. This will turn red whenever turmeric is present, and if an excess of alkali be added it will change to a greenish blue. Evaporate the other half to dryness and moisten with a solution of boric acid and dry on a steam bath. A cherry-red color indicates turmeric.
Martius Yellow or Analogous Coal-Tar Coloring Matter
Extract the slightly acidified sample with 95 per cent alcohol and dye wool as [directed] under “Vegetables.” The wool will be dyed a bright yellow.
Allen’s Test.—Treat a portion of the sample with cold alcohol, and shake vigorously for 5 minutes, then filter and evaporate the filtrate to dryness; add enough water to take up the residue and dye some white wool in this liquid as in the last test. When the dyed wool is wrapped in white paper and heated to 120° in an air bath, part of the coloring matter will be transferred to the paper. The coloring matter dissolves readily in dilute ammonia or hot water, and on the addition of hydrochloric acid the solution is decolorized and a yellow precipitate formed. This distinguishes it from picric acid.
Cayenne Pepper
Allen’s Test.—Boil 1 gram of the mustard for a few minutes with alcohol, filter, and evaporate to dryness at about 100°. Taste the residue and cayenne may be recognized by its pungency. Or heat a portion of the extract, and smell the fumes. Irritation of the lungs and coughing will surely follow if cayenne pepper is present.