OF THE MANAGEMENT OF THE LIGHT.
The pleasure arising from a just view of a microscopic object, the distinctness of vision, &c. depend on a due management of the light, and adapting the quantity of it to the nature of the object, and the focus of the magnifier; therefore, an object should always be viewed in various degrees of light. It is difficult to distinguish in some objects between a prominency and a depression, between a shadow and a black stain; and in colour, between a reflection and a whiteness; a truth which the reader will find fully exemplified in the examination of the eye of the libellula, and other flies, which will be found to appear exceedingly different in one position of the light from what they do in another.
The brightness of an object depends on the quantity of light; the distinctness of vision, on regulating the quantity to the object; for some will be lost and drowned, as it were, in a quantity of light that is scarce sufficient to render another visible, as a different portion of light under the same apparatus will often exhibit in perfection, or totally conceal an object in the substance to be examined. This is more particularly the case with the animalculæ infusoriæ, whose thin and transparent form blend as it were with the water in which they swim; the degree of light must therefore be suited to the object, which, if dark, will be seen best in a strong and full light, but if very transparent, it should be examined in a fainter.
A strong light may be thrown on an object various ways: first, by means of the sun and a convex lens; for this purpose, place the microscope about three feet from a southern window; take a deep convex lens, that is mounted in a semicircle and fixed on a stand, so that its position may be easily varied; place this lens between the object and the window, so that it may collect a considerable number of the solar rays, and refract them on the object, or the mirror of the microscope. If the light thus collected from the sun be too powerful, it may be tempered by placing a piece of oil paper, or a glass lightly greyed, between the object and the lens: by these means, a convenient degree of light may be obtained, and diffused in an equal manner over the whole surface of an object, a circumstance that should be particularly attended to; for if the light be thrown in an irregular manner, that is, larger portions of it on some parts than on others, it will not be distinctly exhibited.
Where the solar light is preferred, it will be found very convenient to darken the room, and to reflect the rays of the sun on the above mentioned lens, by means of the mirror of a solar microscope fitted to the window-shutter; for, by this apparatus, the observer will be enabled to preserve the light on his object, notwithstanding the motion of the sun.
Cutting off the adventitious light as much as possible, by darkening the room where you are using the microscope, and admitting the light only through a hole in the window-shutter, or at most, keeping one window only open, will also be found very conducive towards producing a distinct view of the object.
As the motion of the sun, and the variable state of our atmosphere, render solar observations both tedious and inconvenient, it will be proper for the observer to be furnished with a large tin lanthorn, made something like the common magic lanthorn, fit to contain one of Argand’s lamps.[41] The lanthorn should have an aperture in front, that may be moved up and down, and capable of holding a lens; by this a pleasing uniform dense light may be easily procured. The lamp should move on a rod, that it may be readily elevated or depressed. The lanthorn may be used for many other purposes, as for viewing of pictures, exhibiting microscopic objects on a screen, &c.
[41] The lamp should not be of the fountain kind, because the rarefaction of the air in the lanthorn will often force the oil over.
Many transparent objects are seen best in a weak light; among these we may place the prepared eyes of flies and animalculæ in fluids; the quantity of light from a lamp or candle may be lessened by removing the microscope to a greater distance from them, or it may be more effectually lessened by cutting off a part of the cone of rays that fall on the object, either by placing the cone, as already described with the apparatus to different microscopes, under the stage, or by forming circular apertures of black paper of different sizes, and placing either a large or small one on the reflecting mirror, as occasion may require.
There is an oblique position of the mirrors, and consequently of the light, which is easily acquired by practice, but for which no general rule can be given, that will exhibit an object more beautifully and more distinctly than any other situation, shewing the surface, as well as those parts through which the light is transmitted.
A better view of most objects is obtained by a candle or lamp than by day-light; it is more easy to modify the former than the latter, and to throw it on the object with different degrees of density. From what has been said, the reader will have observed the importance of being able to examine the object in the greatest variety of positions and appearances, which cannot be effected with equal convenience by any microscope, but the improved lucernal.