Experiment No. 12
Testing Olive Oil for Cotton Seed Oil
Pour into a test tube 5 cc. of the oil to be tested and 5 cc. of Halphen's Reagent. Mix thoroughly. Plug the test tube loosely with cotton, and heat in a bath of boiling saturated brine for 15 minutes. If cotton seed oil is present, a deep red or orange color is produced. Test two samples and compare.
Halphen's Reagent.—Mix equal volumes of amyl alcohol and carbon disulphid containing about one per cent of sulphur in solution.
(Adapted from Leach, "Food Inspection and Analysis.")