RESPECT BEES SHOW THEIR QUEEN
When a fertile queen moves about the combs her subjects always open a way for her to pass, and the bees seem to vie with each other in the respect they show their ruler.
The virgin queen never has much respect shown her. The workers do not even trouble themselves to get out of her way when she moves about the cluster. She must run over the bees and get about the best she can.
When a hive is opened and combs removed, a virgin queen is pretty sure to take wing, especially if the operation of removing the frames is not performed quietly, or late in the day. However, there is no danger of the queen being lost as she will fly but a short distance from the hive and immediately return.