Euphoria inda (Family Scarabæidæ).

Of Euphoria inda only one male was captured, but the numerous testes furnished abundant material in desirable stages. The spermatogonial equatorial plate (fig. 107) contains 20 chromosomes of which the two smallest (l and s) form the unequal pair. The resting spermatogonium contains a two-lobed plasmosome (fig. 108). The growth stages are similar to those in Tenebrio in showing no distinct bouquet stage, but there is a spireme stage in which the heterochromosome pair is clearly seen (fig. 109). Figure 110 (plate XI) is an early prophase, and figure 111 one in which the unequal pair appears with a tetrad and several dumb-bell forms. The prophase of the spindle, as in Odontota, is much elongated (fig. 112). In figures 113-116 the small heterochromosome pair is shown in various positions with reference to the other chromosomes of the metaphase of the first spermatocyte. Figure 117 shows it more deeply stained than the others in the equatorial plate. This pair divides in advance of the others, and the larger and smaller elements are plainly seen nearer the poles in anaphase than the other univalent chromosomes (figs. 118-120). Daughter plates of the first spermatocyte are shown in figure 121, and equatorial plates of the second spermatocyte in figure 123. Figure 122 shows the telophase of the first division with the spindle for the second division forming. In figures 124 and 125 we have daughter plates of the two classes of second spermatocytes, showing the content of the two equal classes of dimorphic spermatozoa, as this was shown in Tenebrio. Figures 126 and 127 are anaphases showing the division of the heterochromosomes (l and s). Figures 128-130 are early stages in the development of the spermatid showing the chromatin nucleolus (n) in various phases.