Harvard Psychological Studies, Volume 2

by Unknown

EXPERIMENTAL

In the investigations to be described, 58 crayfish of the species Cambarus affinis were made use of, and for identification the animals were marked on the back with white enamel paint, the males receiving the even numbers from 2 to 64, the females the odd numbers from 1 to 51.

1. Reactions to White Light

The questions proposed for investigation were, (a) How does the crayfish react to diffuse daylight; (b) to reflected sunlight; (c) to direct sunlight; (d) to artificial light of different intensities? (e) What is the influence of previous conditions of exposure to light upon the reactions of the animal? (f) Do changes of temperature affect the reactions?

A wooden box, 80 cm. long, 25 cm. wide, and 20 cm. high, painted black on the inside, and constructed so as to hold water, was covered with a heavy black cloth to exclude the light from above. The front end of the box was of glass, thus admitting the light from the end. In all the experiments except those with direct sunlight, this glass end was covered with black cardboard in which a hole 10 cm. long and 5 cm. high had been so cut that the light was admitted at the middle of the bottom of the glass. The direct sunlight was admitted through the whole of the glass end. At the rear of the box a piece of black cardboard was so arranged that an aperture was afforded for observing the animals without admitting any appreciable amount of light, and this aperture could be readily closed by a slide when not in use.

The method of experimentation was to place the animal in the box about 20 cm. from the glass end, and observe whether it went toward or away from the source of light. The animals were experimented on in two groups of five each, and one hundred observations were made on the individuals of each group with each intensity of light, that is, twenty observations on each animal. In order to check the influence of the orientation of the animal at the time of exposure to the stimulus, the following four positions for placing the animal were chosen: (1) Head toward the light; (2) Head away from the light; (3) At right angles to the light with right side toward it; (4) At right angles with the left side toward the light. Thus five observations were made on each animal of each group in each position, exposed to each of the different intensities of light.

Seven different intensities of light were employed, and the results have been arranged in eight sets, as follows: I. Diffuse daylight in dry box, i. e., the animals were taken out of their ordinary medium, water, and were exposed to the stimulus of diffuse daylight in the air. The reactions under these conditions, however, were so slow and so unsatisfactory that the test was abandoned after the first group, and thus the second group has nothing to show for itself under this head. The remaining seven sets of observations were made on animals placed in 10 cm. of water at 15° C. II. Diffuse daylight. III. Reflected sunlight. The box was placed near a window on a clear day, and the sunlight was thrown in horizontally by means of a mirror. IV. Direct sunlight. On a clear day the box was placed in such a position that the sun shone in directly and illuminated the front half of it. V. 9-candle-power incandescent electric light. This lamp was marked 16 c., but it had been used a great deal, and on being tested with a Lummer-Brodhun photometer showed only 9 c. VI. An incandescent electric light of about 50 c. This lamp was marked 100 c., but had been used considerably and was slightly smoked. Unfortunately it was broken before there was any opportunity to test it. Judging from the fact that another 100 c. lamp of the same manufacture, in slightly better condition, measured 64 c., the estimate of 50 c. seemed a safe one. VII. The incandescent electric light alluded to above, which measured 64 c. VIII. An arc light which varied in intensity from 150 c. to 250 c.

In intensities V and VI the lamp was placed 5 cm. from the glass end of the box to allow the interposition of a heat-screen consisting of an alum solution in a flat glass jar 5 cm. thick. Reckoned in candle-metres, therefore, the intensity of the illumination at the surface of the animal in V was 144 c. m., and that in VI was about 800 c. m. In VII two heat-screens were used, and between these was placed a lens of considerable but not accurately determined curvature, so that it is impossible to express the intensity in candle-metres. In VIII the light was so variable that such an expression would mean nothing.

Unfortunately it was impossible to keep the two groups constant throughout the whole series, owing to the death of two individuals in each group during the experimentation. Group 1 was composed of nos. 1, 3, 4, 8, and 9, of which 1 and 8 were replaced by nos. 13 and 42 respectively. Group 2 was begun with nos. 23, 27, 32, 34, and 38, and the vacancies caused by the death of 23 and 32 were filled by nos. 21 and 36. The following table exhibits the reactions to the different intensities of light, + indicating an orientation toward the source of light, - an orientation away from the light, and ± an indifferent orientation, which usually means no movement at all.