REPRODUCTION IN VAUCHERIA.
After the plant has attained a certain stage in its growth, if it be attentively watched, a marked change will be observed near the ends of the filaments. The chlorophyl appears to assume a darker hue, and the granules become more densely crowded. This appearance increases until the extremity of the tube appears almost swollen. Soon the densely congregated granules at the extreme end will be seen to separate from the endochrome of the filament, a clear space sometimes, but not always, marking the point of division. Here a septum or membrane appears, thus forming a cell whose length is about three or four times its width, and whose walls completely inclose the dark green mass of crowded granules (Fig. 1, b). These contents are now gradually forming themselves into the spore or "gonidium," as Carpenter calls it, in distinction from the true sexual spores, which he terms "oospores." At the extreme end of the filament (which is obtusely conical in shape) the chlorophyl grains retract from the old cellulose wall, leaving a very evident clear space. In a less noticeable degree, this is also the case in the other parts of the circumference of the cell, and, apparently, the granular contents have secreted a separate envelope entirely distinct from the parent filament. The grand climax is now rapidly approaching. The contents of the cell near its base are now so densely clustered as to appear nearly black (Fig. 1, c), while the upper half is of a much lighter hue and the separate granules are there easily distinguished, and, if very closely watched, show an almost imperceptible motion. The old cellulose wall shows signs of great tension, its conical extremity rounding out under the slowly increasing pressure from within. Suddenly it gives way at the apex. At the same instant, the inclosed gonidium (for it is now seen to be fully formed) acquires a rotary motion, at first slow, but gradually increasing until it has gained considerable velocity. Its upper portion is slowly twisted through the opening in the apex of the parent wall, the granular contents of the lower end flowing into the extruded portion in a manner reminding one of the flow of protoplasm in a living amoeba. The old cell wall seems to offer considerable resistance to the escape of the gonidium, for the latter, which displays remarkable elasticity, is pinched nearly in two while forcing its way through, assuming an hour glass shape when about half out. The rapid rotation of the spore continues during the process of emerging, and after about a minute it has fully freed itself (Fig 1, a). It immediately assumes the form of an ellipse or oval, and darts off with great speed, revolving on its major axis as it does so. Its contents are nearly all massed in the posterior half, the comparatively clear portion invariably pointing in advance. When it meets an obstacle, it partially flattens itself against it, then turns aside and spins off in a new direction. This erratic motion is continued for usually seven or eight minutes. The longest duration I have yet observed was a little over nine and one-half minutes. Hassall records a case where it continued for nineteen minutes. The time, however, varies greatly, as in some cases the motion ceases almost as soon as the spore is liberated, while in open water, unretarded by the cover glass or other obstacles, its movements have been seen to continue for over two hours.
The motile force is imparted to the gonidium by dense rows of waving cilia with which it is completely surrounded. Owing to their rapid vibration, it is almost impossible to distinguish them while the spore is in active motion, but their effect is very plainly seen on adding colored pigment particles to the water. By subjecting the cilia to the action of iodine, their motion is arrested, they are stained brown, and become very plainly visible.
After the gonidium comes gradually to a rest its cilia soon disappear, it becomes perfectly globular in shape, the inclosed granules distribute themselves evenly throughout its interior, and after a few hours it germinates by throwing out one, two, or sometimes three tubular prolongations, which become precisely like the parent filament (Fig 2).
Eminent English authorities have advanced the theory that the ciliated gonidium of Vaucheria is in reality a densely crowded aggregation of biciliated zoospores, similar to those found in many other confervoid algæ. Although this has by no means been proved, yet I cannot help calling the attention of the members of this society to a fact which I think strongly bears out the said theory: While watching a gathering of Vaucheria one morning when the plant was in the gonidia-forming condition (which is usually assumed a few hours after daybreak), I observed one filament, near the end of which a septum had formed precisely as in the case of ordinary filaments about to develop a spore. But, instead of the terminal cell being filled with the usual densely crowded cluster of dark green granules constituting the rapidly forming spore, it contained hundreds of actively moving, nearly transparent zoospores, and nothing else. Not a single chlorophyl granule was to be seen. It is also to be noted as a significant fact, that the cellulose wall was intact at the apex, instead of showing the opening through which in ordinary cases the gonidium escapes. It would seem to be a reasonable inference, I think, based upon the theory above stated, that in this case the newly formed gonidium, unable to escape from its prison by reason of the abnormal strength of the cell wall, became after a while resolved into its component zoospores.