This sub-class is represented by a single family (Cirrostomi) and by a single genus (Branchiostoma);[48] it is the lowest in the scale of fishes, and lacks so many characteristics, not only of this class, but of the vertebrata generally, that Hæckel, with good reason, separates it into a separate class, that of Acrania. The various parts of its organisation have been duly noticed in the first part of this work.

The “Lancelet” (Branchiostoma lanceolatum, see Fig. [28], p. 63), seems to be almost cosmopolitan within the temperate and tropical zones. Its small size, its transparency, and the rapidity with which it is able to bury itself in the sand, are the causes why it escapes so readily observation, even at localities where it is known to be common. Shallow, sandy parts of the coasts seem to be the places on which it may be looked for. It has been found on many localities of the British, and generally European coasts, in North America, the West Indies, Brazil, Peru, Tasmania, Australia, and Borneo. It rarely exceeds a length of three inches. A smaller species, in which the dorsal fringe is distinctly higher and rayed, and in which the caudal fringe is absent, has been described under the name of Epigionichthys pulchellus; it was found in Moreton Bay.

APPENDIX.

DIRECTIONS FOR COLLECTING AND PRESERVING FISHES.

Whenever practicable fishes ought to be preserved in spirits.

To insure success in preserving specimens the best and strongest spirits should be procured, which, if necessary, can be reduced to the strength required during the journey with water or weaker spirit. Travellers frequently have great difficulties in procuring spirits during their journey, and therefore it is advisable, especially during sea voyages, that the traveller should take a sufficient quantity with him. Pure spirits of wine is best. Methylated spirits may be recommended on account of their cheapness; however, specimens do not keep equally well in this fluid, and very valuable objects, or such as are destined for minute anatomical examination, should always be kept in pure spirits of wine. If the collector has exhausted his supply of spirits he may use arrack, cognac, or rum, provided that the fluids contain a sufficient quantity of alcohol. Generally speaking, spirits which, without being previously heated, can be ignited by a match or taper, may be used for the purposes of conservation. The best method to test the strength of the spirits is the use of a hydrometer. It is immersed in the fluid to be measured, and the deeper it sinks the stronger is the spirit. On its scale the number 0 signifies what is called proof spirit, the lowest degree of strength which can be used for the conservation of fish for any length of time. Spirits, in which specimens are packed permanently, should be from 40 to 60 above proof. If the hydrometers are made of glass they are easily broken, and therefore the traveller had better provide himself with three or four of them, their cost being very trifling. Further, the collector will find a small distilling apparatus very useful. By its means he is able not only to distil weak and deteriorated spirits or any other fluid containing alcohol, but also, in case of necessity, to prepare a small quantity of drinkable spirits.

Of collecting vessels we mention first those which the collector requires for daily use. Most convenient are four-sided boxes made of zinc, 18 in. high, 12 in. broad, and 5 in. wide. They have a round opening at the top of 4 in. diameter, which can be closed by a strong cover of zinc of 5 in. diameter, the cover being screwed into a raised rim round the opening. In order to render the cover air-tight, an indiarubber ring is fixed below its margin. Each of these zinc boxes fits into a wooden case, the lid of which is provided with hinges and fastenings, and which on each side has a handle of leather or rope, so that the box can be easily shifted from one place to another. These boxes are in fact made from the pattern of the ammunition cases used in the British army, and extremely convenient, because a pair can be easily carried strapped over the shoulders of a man or across the back of a mule. The collector requires at least two, still better four or six, of these boxes. All those specimens which are received during the day are deposited in them, in order to allow them to be thoroughly penetrated by the spirit, which must be renewed from time to time. They remain there for some time under the supervision of the collector, and are left in these boxes until they are hardened and fit for final packing. Of course, other more simple vessels can be used and substituted for the collecting boxes. For instance, common earthenware vessels, closed by a cork or an indiarubber covering, provided they have a wide mouth at the top, which can be closed so that the spirit does not evaporate, and which permits of the specimens being inspected at any moment without trouble. Vessels in which the objects are permanently packed for the home journey are zinc boxes of various sizes, closely fitting into wooden cases. Too large a size should be avoided, because the objects themselves may suffer from the superimposed weight, and the risk of injury to the case increases with its size. It should hold no more than 18 cubic feet at most, and what, in accordance with the size of the specimens, has to be added in length should be deducted in depth or breadth. The most convenient cases, but not sufficient for all specimens, are boxes 2 feet in length, 1½ foot broad, and 1 foot deep. The traveller may provide himself with such cases ready made, packing in them other articles which he wants during his journey; or he may find it more convenient to take with him only the zinc plates cut to the several sizes, and join them into boxes when they are actually required. The requisite wooden cases can be procured without much difficulty almost everywhere. No collector should be without the apparatus and materials for soldering, and he should be well acquainted with their use. Also a pair of scissors to cut the zinc plates are useful.

Wooden casks are not suitable for the packing of specimens preserved in spirits, at least not in tropical climates. They should be used in cases of necessity only, or for packing of the largest examples, or for objects preserved in salt or brine.

Very small and delicate specimens should never be packed together with larger ones, but separately, in small bottles.

Mode of preserving.—All fishes, with the exception of very large ones (broad kinds exceeding 3–4 feet in length; eel-like kinds more than 6 feet long), should be preserved in spirits. A deep cut should be made in the abdomen between the pectoral fins, another in front of the vent, and one or two more, according to the length of the fish, along the middle line of the abdomen. These cuts are made partly to remove the fluid and easily decomposing contents of the intestinal tract, partly to allow the spirit quickly to penetrate into the interior. In large fleshy fishes several deep incisions should be made with the scalpel into the thickest parts of the dorsal and caudal muscles, to give ready entrance to the spirits. The specimens are then placed in one of the provisional boxes, in order to extract, by means of the spirit, the water of which fishes contain a large quantity. After a few days (in hot climates after 24 or 48 hours) the specimens are transferred into a second box with stronger spirits, and left therein for several days. A similar third and, in hot climates sometimes a fourth, transfer is necessary. This depends entirely on the condition of the specimens. If, after ten or fourteen days of such treatment the specimens are firm and in good condition, they may be left in the spirits last used until they are finally packed. But if they should be soft, very flexible, and discharge a discoloured bloody mucus, they must be put back in spirits at least 20° over proof. Specimens showing distinct signs of decomposition should be thrown away, as they imperil all other specimens in the same vessel. Neither should any specimen in which decomposition has commenced when found, be received for the collecting boxes, unless it be of a very rare species, when the attempt may be made to preserve it separately in the strongest spirits available. The fresher the specimens to be preserved are, the better is the chance of keeping them in a perfect condition. Specimens which have lost their scales, or are otherwise much injured, should not be kept. Herring-like fishes, and others with deciduous scales, are better wrapped in thin paper or linen before being placed in spirits.