Hæmatoxylin and eosin can also be combined in one stain, but the results are not as good as those obtained by successive staining.
b. Haematoxylin and Picric Acid.
1. Stain with Weigert’s iron-hæmatoxylin, or overstain with any other hæmatoxylin.
2. Wash thoroughly.
3. Stain in saturated water solution of picric acid, diluted one-half, until sections are a bright yellow. If left too long in the stain, the hæmatoxylin will become brown or may be wholly lost.
4. Wash, dehydrate and clear quickly, as for eosin. (Dry picric acid may be added to the carbol-xylol.)
5. Mount in balsam.
c. Haematoxylin and Acid Fuchsin.
Stain with hæmatoxylin, and after washing use a 1 per cent water solution of acid fuchsin, until section is sufficiently red; wash; dehydrate; clear; mount.
d. Haematoxylin and Orange G.