Nuclei are green; Russell’s fuchsin-bodies light-red; Altmann’s granules light-red.
3. Pianese’s Method.
1. Fix in Pianese’s solution (see methods of fixation) 6 hours; wash in running water for 12 hours; after-harden in graded alcohols; imbed in paraffin; mount on cover-glass.
2. Stain 30 minutes in a staining mixture consisting of malachite green 0.5 grm., acid fuchsin 0.1 grm., Martius yellow 0.01 grm., distilled water 150 cc., 96 per cent alcohol 50 cc.
3. Dehydrate in absolute alcohol; clear in xylol; balsam.
Nuclei are green; protoplasm reddish; cell-inclusions light-red.
4. Method for Staining “Plimmer’s Bodies.”
1. Fix in Hermann’s fluid for 12-24 hours. Imbed in paraffin. Mount sections on cover or slide.
2. Transfer sections to hydrogen peroxide for 15-30 seconds.
3. Wash in water.