1. Buzzi’s Method of Staining Eleidin and Keratohyalin.
1. Harden, imbed, cut.
2. Stain in Congo red (2-3 drops of a 1 per cent water solution added to small basin of water) for 2-3 minutes.
3. Wash thoroughly in water.
4. Stain in hæmatoxylin, and wash.
5. Dehydrate in absolute alcohol; xylol; balsam.
Keratohyalin blue, eleidin red.
2. Fick’s Method of Staining Keratohyalin and Keratin.
1. Harden in alcohol, imbed, cut.
2. Stain in saturated water solution of kresyl-echt-violett for 3-4 minutes.