5. 80 per cent alcohol ½-2 hours.
6. Wash in water.
7. Place section flat on slide; blot; add a drop of warmed glycerin-gelatin; cover quickly. Ringing or sealing is not necessary.
Or, to mount section in balsam:—
After 6, counterstain with hæmatoxylin or safranin; wash again; dehydrate quickly with absolute alcohol; clear in pure benzene; mount in pure melted Canada balsam (containing no xylol).
2. Staining of Fat with Sudan III or Scharlach R.
Staining-solutions of these dyes may be made, as follows:—
a. Dissolve stain in 70-80 per cent boiling alcohol, keep in the incubator over night, and use warm.
b. Make a solution of absolute alcohol 70 cc., 10 per cent caustic soda solution 20 cc., water 100 cc. Saturate this with the stain, slightly heating.
c. Make a mixture of 70 per cent alcohol 50 cc. and pure acetone 50 cc.; saturate this with the stain.