XIII. HYDROPIC DEGENERATION. Fix by heat or formol-alcohol. Imbed in celloidin; stain with hæmatoxylin and eosin.
XIV. HYPERTROPHY. Fix in Müller’s or mercuric chloride for simple staining; for study of nuclei fix in Flemming’s and stain with safranin.
XV. INFLAMMATION. The process of inflammation may be studied to advantage in the mesentery, web or tongue of the curarized living frog, by stretching these parts over a cork-ring attached to a glass plate on which the animal rests. The exposed tissues must be kept moist with physiologic salt solution. Heat, chemicals or other irritants may be employed to produce the inflammatory reaction. For the study of the inflammatory process in sections the ordinary fixations may be employed, but for the study of the nuclei, mitotic figures and cell-granulations fixation in Flemming’s, Zenker’s, etc., is advised. Safranin, methylene blue and eosin, the various stains used in the study of blood-cells, etc., may be used.
1. Staining of Mast-cells.
a. Kresyl-echt-violett used as for amyloid or mucin is the best stain for mast-cells. The cell-granules stain bright rose-red.
b. Ehrlich’s Dahlia Method.
1. Harden in absolute alcohol; imbed; cut.
2. Stain with saturated water solution of dahlia.
3. Wash in water.
4. Dehydrate in absolute alcohol.