5. Acid alcohol until iron-pigment becomes blue (½-12 hours). Complete differentiation of nuclei.
6. Wash in water.
7. Dehydrate in absolute alcohol.
8. Clear in xylol; mount in balsam.
Hæmosiderin is blue (Berlin blue); nuclei are red. Lithium carmine may be used after the iron-test, if desired.
2. Ammonium Sulphide Test for Iron.
1. Fix in alcohol; imbed; cut.
2. Treat sections with yellow ammonium sulphide for 5-60 minutes.
3. Wash quickly in water.
4. Dehydrate in absolute alcohol.