1. Fix in a chromic solution. In this the chromophilic cells become yellow or brown.
2. Stain in polychrome methylene blue; the cells become grass-green in color.
5. Tests for Silver, Lead and Mercury.
Use ammonium sulphide as for iron. Black sulphides are formed.
6. Test for Copper.
Treat with potassium ferrocyanid and hydrochloric acid; copper gives a dark yellow-brown coloration.
XXIII. PSEUDOMUCIN. It is not precipitated by acetic acid. It has a greater affinity for the diffuse stains than mucin, and gives weaker metachromatic reactions.
XXIV. REGENERATION AND REPAIR. For the staining of mitoses, cell granules and cell-inclusions see methods given above. See also methods for staining of epithelium, reticulum, neuroglia, etc.
XXV. URIC ACID AND PURIN BASES:—
1. Courmont and Andre’s Method.