3. Wash in water.
4. Differentiate in 1 per cent acetic acid, 10-30 seconds.
5. Wash in 90 per cent alcohol, 2-5 minutes; dehydrate in absolute alcohol; clear in xylol; mount in balsam.
2. Gentian-violet.
1. Stain sections in a 2 per cent watery gentian-violet for 5-20 minutes.
2. Wash in water.
3. Decolorize in 70 per cent alcohol until stain ceases to come away.
4. Dehydrate in absolute alcohol; clear in xylol; balsam.
3. Zieler’s Method.
1. Fix in Orth’s solution, or any fixing solution except those containing osmic acid; imbed in paraffin or celloidin.