5. Wash in physiologic saline solution.
6. Transfer sections to Lugol’s for 1 minute.
7. Pass rapidly through water.
8. Dry thoroughly between folds of filter-paper.
9. Cover section with aniline-oil until clear.
10. Xylol; balsam.
Clubs pink; mycelium blue.
2. Nocardia, Cladothrix, Streptothrix and Leptothrix. Löffler’s methylene-blue and carbol-fuchsin give good results. The Nocardiæ are acid-fast with dilute acids. They give good preparations with Weigert’s fibrin stain and lithium-carmine.
d. Vibrios.
1. Cholera Vibrios. Gram-negative. Sections may be stained with fuchsin or methylene-blue.