2. Decalcify in Ebner’s hydrochloric acid solution. Wash thoroughly. After-harden in alcohol. Imbed in celloidin or paraffin. Cut very thin sections.
3. Water for 10 minutes.
4. Stain in the alkaline-thionin solution, as in previous method, for 3 minutes.
5. Wash in water.
6. With glass needles transfer sections to a saturated watery solution of phosphotungstic or phosphomolybdic acid for a few seconds.
7. Wash 5-10 minutes, until section is sky-blue in color.
8. Fix the stain for 3-5 minutes in ammonia 1 part, water 10 parts.
9. Transfer directly to 90 per cent alcohol; change twice.
10. Dehydrate; clear in carbol-xylol; balsam.
If the ground-substance is too dark differentiate in acid alcohol before dehydrating, and then wash thoroughly before beginning the dehydration.