1. Harden in absolute alcohol; imbed; cut.
2. Stain 5-15 minutes in polychrome methylene-blue.
3. Wash in water.
4. Differentiate in 1 per cent neutral orcein in absolute alcohol, 15 minutes.
5. Dehydrate in absolute alcohol.
6. Clear in xylol; mount in balsam.
Nuclei dark blue; protoplasm light blue; collagen dark red; plasma-cell granules greenish-blue; mast-cell granules red.
4. Mallory’s Fibroglia Method.
1. Fix thin, small, fresh pieces of tissue in Zenker’s fluid; harden in alcohol; imbed in celloidin or paraffin; cut.
2. Stain sections in 1 per cent aqueous acid fuchsin for 12 hours in the cold, or 20-30 minutes at 50-56°C.