3. Stain in the resorcin-fuchsin mixture for 20-60 minutes.
4. Wash rapidly in acid alcohol.
5. Dehydrate and differentiate in absolute alcohol until section is red.
6. Clear in xylol; balsam.
Nuclei are red; elastic fibres blue-black. Should the stain when old give a diffuse staining differentiate for a longer time in acid alcohol.
c. Fat Tissue. Use same methods as advised for the demonstration of fatty degeneration and infiltration (osmic acid, scharlach R, sudan III).
V. EAR.
Remove temporal bone; fix in formol-Müller’s; decalcify in trichloracetic acid; wash; after-harden in alcohol; imbed in celloidin. For nerve-endings use Golgi’s and methylene-blue methods.
VI. EYE.
Fix in Müller’s, formol-Müller’s, Zenker’s, formol, Flemming’s or Marchi’s solution. Aid fixation by incisions into sclera. The eye should not be left in formol for more than 3 days. Section as desired; imbed larger pieces in celloidin, small ones in paraffin. Use alum-carmine, iron-hæmatoxylin, Van Gieson’s, Weigert’s elastic stain, Levaditi’s silver-method, Golgi’s nerve-stain, methylene-blue method, etc., according to the object of the investigation.