| Sol. III.— | Glycerin | 400 | cc. |
| Water | 2,000 | cc. | |
| Potassium acetate | 200 | grms. |
The specimens must be kept in air-tight jars, and crystals of thymol added to prevent growth of moulds. This is sometimes very difficult, and it becomes necessary to change the discolored fluid for clear. I have found the rectangular museum jars best adapted for the preservation of Kaiserling specimens. I use a wooden top which fits over a thick piece of felt cut just the size of the jar, which in turn fits over a piece of dental rubber cut to fit the jar. The jar is placed upon a wooden bottom which has upright steel rods at the corners, that pass through holes in the wooden top, and have a screw-thread so that they can be fitted with screws, which when screwed down hold the wooden top, felt and rubber sheeting tightly in place, making the jar air-tight, but giving a top easily removable. Very beautiful specimens can be secured by the Kaiserling method, and they can be kept for several years, but sooner or later the color-effect is lost. Light, heat and exposure to the air cause a loss of color.
Some workers prefer the following in place of Sol. I:—
| Hot water | 2,000 | cc. |
| Sodium sulphate | 40 | grms. |
| Magnesium sulphate | 40 | grms. |
| Sodium chloride | 20 | grms. |
When salts are dissolved and solution cool add 200 cc. of formalin.
Melnikow-Raswedenkow Method:—
| Sol. I.— | Water | 100 | parts |
| Formol | 10 | parts | |
| Sodium acetate | 3 | parts | |
| Potassium chlorate | 0.5 | part |
Leave in this 1-2-3-4-5 days, according to size of specimen. Large organs must have solution injected into vessels.
Sol. II.— 95 per cent alcohol, until color is restored.
| Sol. III.— | Preserve in: Water | 100 | parts |
| Glycerin | 60 | parts | |
| Potassium acetate | 30 | parts |