3. Dehydrate in 80 and 95 per cent alcohols; clear in carbol-xylol: mount in balsam. Nuclei are a light reddish violet; the plasma is slightly stained (muscle) or not at all.
b. Lithium Carmine.
2-5 grms. of carmine to 100 cc. of a cold saturated water solution of lithium carbonate; filter.
1. Stain 1-3 minutes; transfer directly to acid alcohol (1 cc. of hydrochloric acid to 100 cc. of 70 per cent alcohol) without putting section into water; differentiate ¼-6 hours, until nuclei alone retain the color. Control differentiation by examination on the slide in acid alcohol.
2. Wash thoroughly in water.
(Use plasma stain, as picric acid, if desired.)
3. Deyhdrate in 80 and 95 per cent alcohols; clear in carbol-xylol; mount in balsam.
c. Borax Carmine.
Dissolve by boiling 0.5-0.75 grm. carmine and 1-2 grms. of borax in 100 cc. of water. To the hot solution add 5 cc. of a O.5 per cent acetic acid until solution is deep red. After 24 hours filter and add crystal of thymol.
Stain and differentiate as with lithium-carmine, but leave the sections somewhat longer in the stain (15 minutes).