During succeeding years the influence of Byzantine womanhood and the relations between the two kingdoms continued prominent. John died in 1297, leaving two sons, Alexius II. and Michael. The former succeeded his father at the age of fifteen, and was placed under the guardianship of his mother Eudocia's brother, the Byzantine emperor Andronicus II. Andronicus ordered his ward, the young emperor of Trebizond, though an independent sovereign prince, to marry Irene, the daughter of a Byzantine subject, Choumnus, one of his favorite ministers. But the idea of a Comnenus marrying below his station was offensive both to Alexius and his people. In obedience to the blood within his veins, and in contempt of his guardian's command, Alexius rejected the proposed mesalliance, and married the daughter of an Iberian prince.

The young married couple presented a beautiful example of conjugal tenderness and devotion, but this did not soften the hard heart of the guardian. Andronicus even went so far as to endeavor, to make the Greek Church declare the marriage null and void on the ground that it had been contracted by a union without the consent of his guardian. But the patriarch and clergy, sympathizing with the lovers, and alarmed at the ludicrous position in which they would be placed, took advantage of the interesting condition of the bride to refuse to gratify the spleen of the chagrined emperor.

At this time also, Eudocia, the mother of Alexius, who was in partial durance in the imperial palace at Constantinople, saw an opportunity of obtaining her freedom and of returning to her dominions. Her brother Andronicus was offended with her because she had rejected his proposal to form a second marriage with the Krai of Servia.

She persuaded her brother that her influence over her son, who was devotedly attached to her, would have far more weight in making the young emperor agree to a divorce than the sentence of an ecclesiastical tribunal whose authority he was able to decline; and to this end she obtained her brother's permission to return to Trebizond. Upon arriving at her son's court Eudocia was so much impressed with the conjugal fidelity of her son Alexius that she at once approved of his conduct, and supported him in his determination to resist the tyrannical pretensions of his guardian. Eudocia is an excellent example of the superiority of the Palæologi women over their weaker and more selfish brothers. In every situation, even in her months of exile from her dominions, she maintained herself with dignity, and in her careful rearing of her son and regard for his interests she exhibited motherly traits of a high order.

In the next generation there was also an alliance between the royal families of the two kingdoms. The emperor Basilius, second son of Alexius II., married Irene Palæologina, the natural daughter of Andronicus III. of Constantinople. Basilius had no legitimate issue, but falling in love with a beautiful lady of Trebizond, also named Irene, he made her his mistress and conferred on her every possible honor. She bore him four children. To insure the succession of one of his natural sons, Basilius in 1339 persuaded or forced the clergy to celebrate a public marriage with his Trebizontine mistress, though there is no evidence that he obtained a divorce from his lawful wife Irene, beyond his own decree. He died suddenly in the April following his marriage to his mistress.

Irene Palæologina, who was, in spite of his second nuptials, universally regarded as the lawful wife of Basilius, was suspected of having hastened his end; and her unfaithful husband had certainly tried the soul of the proud lady. At any rate she was prepared for the sad event, and had already organized a faction which placed her on the throne, as the second independent Empress of Trebizond.

This promptitude in profiting by her husband's death, was worthy of the first Empress Irene in Byzantine history, and gave just ground for suspicion. But in considering an age when it was usual for people to circulate calumnious reports against their rulers, the evidence should be strong before we condemn the Palæologi princess. However, the flagrant immorality of the court circles, and the lightness of character of Irene herself, as well as her conduct after the event, tended to give credibility to the rumor.

Irene, as soon as she was safely established on the throne, sent off her rival of Trebizond and the two sons of Basilius to Constantinople where her father Andronicus detained them as hostages for the tranquillity of her empire. A strong party of the nobility, however, who had hoped to gain wealth and power through the favor of the Trebizontine Irene, whom they purposed to make regent during the minority of her children, were chagrined at the success of the schemes of the Palæologi princess, and at once began to plan her downfall. Two great parties arose, and the little empire was once more disturbed by the turmoil of civil war. Irene, with all her daring, was, like her father, of a gay and thoughtless disposition, and did not fully realize the danger of her situation. She recognized, however, that a second husband would strengthen her cause; and she urged her father Andronicus to send her a husband chosen from among the Byzantine nobles, who could aid her in repressing the factions which threatened her throne. Andronicus gave a favorable reception to Irene's ambassadors, but died before he had time seriously to attend to her request. The light-minded Irene consoled herself during the delay by falling in love with the grand domesticos of her palace. But this bit of favoritism only divided her own court into factions and strengthened the cause of her enemies.

A new storm now burst over the head of the thoughtless empress. Another woman, whose title to rule was far stronger than that of Irene, appeared to claim the throne. Anna, called Anachoutlon, was the eldest daughter of the Emperor Alexius II. She had in early womanhood taken the veil, and until this time had lived in seclusion. The opposition party searched out her retreat and persuaded her to quit her monastic dress and escape to Lazia, where she was proclaimed Empress of Trebizond, as the nearest legitimate heir of her brother Basilius. All the provincials united in demanding the sovereignty of a member of the house of Grand-Comnenus in preference to the usurpation of a Palæologi princess, who was planning to marry a foreigner. The popular demand for the rule of a scion of the house of Grand-Comnenus gave Anna a triumphal march to the capital, and with but little opposition she was admitted within the citadel and universally recognized as the lawful empress. Irene was dethroned after a troubled reign of one year and four months. Three weeks later Michael Grand-Comnenus, second son of John II. and Eudocia, who had been selected at Constantinople as a suitable husband of Irene, arrived on the scene, to find the change of sovereignty. The Empress Anna was surrounded by a cabal of powerful chiefs, who determined to keep the reins of power in their hands. She graciously received her kinsman, but he was later treacherously seized and imprisoned by Anna's partisans. Irene was sent on, under suitable escort, to Constantinople, to pass the rest of her life in retirement. The treatment of Michael aroused the fury of many adherents of the house of Grand-Comnenus. Another upheaval followed. John III., son of Michael, was brought over from Constantinople, and proclaimed emperor by a constantly growing faction. The hapless Anna, who had doubtless ofttimes regretted giving up the peaceful life of the monastery for the troubles and cares of a crown, was taken prisoner in the palace, and was immediately strangled. She had occupied the throne hardly more than a year.

The next period of importance in our study of Trebizontine princesses is that covered by the long reign--1349-1390--of Alexius III., the second son of Basilius by Irene of Trebizond. His wife was also a Byzantine princess, Theodora, the daughter of Nicephorus Cantacuzenus, brother of the emperor John V., Cantacuzenus, whose stormy career of opposition to Anne of Savoy we have already noticed. Theodora bore to Alexius a number of beautiful daughters, whom he utilized when they became of marriageable age to form alliances with his powerful neighbors, both Mohammedan and Christian. His eldest daughter, Eudocia, Alexius first wedded to the Emir Tadjeddin, who had gained possession of the important district of Limnia; after Tadjeddin was slain in a quarrel with a neighboring emir, the beautiful and accomplished princess became the wife of the Byzantine emperor, John V. That aged monarch had chosen her to be the bride of his son, the emperor Manuel II.,--Palæologus; but when she arrived at Constantinople for the celebration of the nuptials, her beauty and grace so powerfully captivated the decrepit old debauchee that he set aside the inclinations of his son, who was also enamored of his prospective bride, and married the young widow himself.