It is only after the bed has been emptied that the real activity of the vast number of micro-organisms commences, which is directed towards converting the organic substances into mineral ones. This process of splitting up, decomposing, disintegrating and mineralising organic waste products is an exceedingly complex one, which ever fluctuates according to the prevailing conditions, and which does not come to an end until finally stable mineral forms are reached. In the presence of a plentiful supply of oxygen, the process proceeds as a rule at a more rapid rate, and the intermediate forms produced are less complex than in the comparative or total absence of this gas; hence the progress of the process is largely determined by it. The amount of oxygen necessary for bacterial activity is partly abstracted, and with extraordinary energy, from the atmospheric air in the pores of the filling material, and a portion of the substances formed, such as carbonic acid and nitrogen—in gas form—escape into the atmosphere, whilst the remaining portions are washed out of the bed with other products, such as nitric acid, by the effluent.

Further remarks upon this process of mineralisation have been made in connection with the subject of natural self-purification of sewage, and these may be referred to here.

Effluent from bed practically raw sewage as far as its bacterial contents are concerned.

The effect of the bed upon the bacterial flora of sewage is, as was to be expected, but very slight, and it is on record now that, as far as the micro-organic life is concerned, the effluent is to all intents and purposes raw sewage.

Silting up of bed.

Some of the substances contained in raw sewage remain in the bed, no matter how carefully the sewage has been previously strained, and these, in combination with the slimy surface coating of the component particles, the accumulation of mineralised substances in the pores, the consolidation of the bed, the disintegration of the filling material, and the liquid retained, lead gradually but surely to the silting or sludging up of the bed.

Theoretical original water capacity of bed.

(c) Water Capacity of Bed and Silting up.—The theoretical water capacity of the bed, previous to commencing operations, is the aggregate of the cubical space occupied by the pores or small passages between the particles forming the filling material, and the pores of the filling material itself; but in practice a certain amount of this space is occupied by air, which it is impossible to dislodge altogether in filling. The aggregate of the cubical space of the pores may be called the pore-volume.

It is difficult to lay down general rules as to what the original water capacity of a bed should be expressed in per cent. of the space occupied by the filling material, but speaking within fairly wide limits the following is somewhat near the truth.

Original water capacity with spherical particles of uniform size.