By the American Public Health Association

CONTENTS.

PAGE
Preface to the Fourth Edition[vii]
Collection of Samples[1]
Quantity of Water Required for Analysis[1]
Bottles[1]
Time Interval between Collection and Analysis[2]
Representative Samples[3]
Physical Examination[4]
Temperature[4]
Turbidity[4]
Turbidity Standard[4]
Platinum Wire Method[5]
Turbidimetric Method[7]
Coefficient of Fineness[8]
Color[9]
Comparison with Platinum-Cobalt Standards[9]
Comparison with Glass Disks[10]
Comparison with Nessler Standards[10]
Lovibond Tintometer[11]
Odor[12]
Cold Odor[12]
Hot Odor[12]
Expression of Results[12]
Chemical Examination[14]
Expression of Results[14]
Forms of Nitrogen[15]
Ammonia Nitrogen[15]
Determination by Distillation[15]
Measurement of Ammonia Nitrogen[16]
Comparison with Ammonia Standards[16]
Comparison with Permanent Standards[17]
Modification for Sewage[18]
Determination by Direct Nesslerization[19]
Albuminoid Nitrogen[20]
Organic Nitrogen[21]
Nitrite Nitrogen[22]
Nitrate Nitrogen[23]
Phenoldisulfonic Acid Method[23]
Reduction Method[24]
Total Nitrogen[25]
Oxygen Consumed[25]
Recommended Method[26]
Other Methods[27]
Residue on Evaporation[29]
Total Residue[29]
Fixed Residue and Loss on Ignition[29]
Suspended Matter[30]
Determination with Gooch Crucible[30]
Determination by Filtration[30]
Determination of Volume[30]
Fixed Residue and Loss on Ignition[30]
Hardness[30]
Total Hardness by Calculation[31]
Total Hardness by Soap Method[31]
Total Hardness by Soda Reagent Method[34]
Temporary Hardness by Titration with Acid[34]
Non-Carbonate Hardness by Soda Reagent Method[34]
Non-Carbonate Hardness by Soap Method[35]
Alkalinity[35]
Procedure with Phenolphthalein[36]
Procedure with Methyl Orange[37]
Procedure with Lacmoid[37]
Procedure with Erythrosine[37]
Bicarbonate[37]
Normal Carbonate[38]
Hydroxide[38]
Alkali Carbonates[39]
Acidity[39]
Total Acidity[40]
Free Carbon Dioxide[40]
Free Mineral Acids[41]
Mineral Acids and Sulfates of Iron and Aluminium[41]
Chloride[41]
Iron[43]
Total Iron[44]
Colorimetric Method[44]
Comparison with Iron Standards[45]
Comparison with Permanent Standards[46]
Volumetric Method[46]
Dissolved Iron[47]
Suspended Iron[47]
Ferrous Iron[47]
Ferric Iron[48]
Manganese[48]
Persulfate Method[48]
Bismuthate Method[49]
Lead, Zinc, Copper, and Tin[50]
Lead[51]
Zinc[52]
Copper[53]
Tin[54]
Mineral Analysis[56]
Residue on Evaporation[56]
Alkalinity and Acidity[56]
Chloride[56]
Nitrate Nitrogen[56]
Separation of Silica, Iron, Aluminium, Calcium, and Magnesium[56]
Silica[56]
Iron and Aluminium[57]
Calcium[57]
Magnesium[57]
Separation of Sulfate, Sodium, and Potassium[58]
Sulfate[58]
Sodium, Potassium and Lithium[58]
Potassium[59]
Lithium[60]
Bromine, Iodine, Arsenic, and Boric Acid[61]
Bromine and Iodine[61]
Arsenic[63]
Boric Acid[63]
Hydrogen Sulfide[63]
Chlorine[64]
Dissolved Oxygen[65]
Ether-Soluble Matter[69]
Relative Stability of Effluents[69]
Biochemical Oxygen Demand of Sewages and Effluents[71]
Relative Stability Method[71]
Sodium Nitrate Method[72]
Analysis of Sewage Sludge and Mud Deposits[73]
Collection of Sample[73]
Reaction[73]
Specific Gravity[74]
Moisture[74]
Volatile and Fixed Matter[74]
Total Organic Nitrogen[74]
Ether-Soluble Matter[75]
Ferrous Sulfide[76]
Biochemical Oxygen Demand[76]
Analysis of Chemicals[77]
Reagents[77]
Sulfate of Aluminium[78]
Insoluble Matter[78]
Oxides of Aluminium and Iron[78]
Total Iron[79]
Ferric Iron[79]
Ferrous Iron[80]
Basicity Ratio[80]
Lime[80]
Sulfate of Iron[81]
Insoluble Matter[81]
Iron as Ferrous Sulfate[81]
Acidity[81]
Soda Ash[82]
Insoluble Matter[82]
Available Alkali[82]
Chemical Bibliography[82]
Microscopical Examination[89]
Microscopical Bibliography[91]
Bacteriological Examination[92]
Apparatus[92]
Sample Bottles[92]
Pipettes[92]
Dilution Bottles[92]
Petri Dishes[92]
Fermentation Tubes[92]
Materials[93]
Water[93]
Meat Extract[93]
Peptone[93]
Sugars[93]
Agar[93]
Gelatin[93]
Litmus[93]
General Chemicals[93]
Methods[93]
Preparation of Culture Media[93]
Titration[93]
Sterilization[94]
Nutrient Broth[95]
Sugar Broths[95]
Nutrient Gelatin[95]
Nutrient Agar[96]
Litmus or Azolitmin Solution[96]
Litmus-Lactose-Agar[97]
Endo’s Medium[97]
Collection of Sample[98]
Storage and Transportation of Sample[98]
Dilutions[98]
Plating[99]
Incubation[99]
Counting[99]
The Test for the Presence of Members of the B. Coli Group[100]
Presumptive Test[100]
Partially Confirmed Test[101]
Completed Test[102]
Application of these Tests[102]
Expression of Results[103]
Summary of these Tests[104]
Interpretation of Results[106]
Differentiation of Fecal from Non-fecal Members of the B. Coli Group[106]
Methyl Red Test[107]
Voges-Proskauer Test[108]
Routine Procedure for Bacteriological Examination[108]
Bacteriological Bibliography[110]
Index[113]

PREFACE TO FOURTH EDITION.

The Committee on Standard Methods of Bacteriological Water Analysis was reorganized in 1918 with the following membership: F. P. Gorham, chairman, L. A. Rogers, W. G. Bissell, H. E. Hasseltine, H. W. Redfield, with M. Levine as adjunct member. This committee made a report in 1918 which was not acted on by the Laboratory Section, and in 1919 made a revised report, recommending certain changes in Standard Methods, which were adopted by the section and which are now incorporated in this present fourth edition.

Following are the more important changes:

New brands of peptone authorized.

Phenol Red Method of Hydrogen-ion Concentration.

Five-tenths per cent of sugar specified for broths instead of 1 per cent.