As Darnley was a Catholic, the Protestant party was much alarmed. The lords, headed by Murray, assembled at Stirling, and entered into a bond to stand by each other. They sent off a messenger to urge speedy aid from Elizabeth, and actively diffused reports that Lennox had plotted to take away the life of Murray. This, both Lennox and Darnley stoutly denied, and the queen, to leave no obscurity in the case, gave Murray a safe conduct for himself and eighty others, and ordered him to attend in her presence and produce his proofs. She declared that such a thing as enforcement of the religion or consciences of her subjects had never entered her mind, and she called on her loyal subjects to hasten to her defence. This call was promptly and widely responded to, and Mary, finding herself now in security, declared the choice of Darnley as her husband, created him Duke of Albany, and married him openly, in the chapel of Holyrood. He was by proclamation declared king during the time of their marriage, and all writs were ordered to run in the joint names of Henry and Mary, King and Queen of Scotland.
Elizabeth, meanwhile, had complied with the demands of the Scottish lords; sent off money, appointed Bedford and Shrewsbury her lieutenants in the north, and reinforced the garrison of Berwick with 2,000 men. Finding, however, that the call of Mary on her subjects had brought out such a force around her as would require still more money and men to cope with it, she despatched Tamworth, a creature of Leicester's, to Scotland, to deter Mary by menaces and reproaches. It was too late; and Mary, assuming the attitude of a justly incensed monarch, compelled the ambassador to deliver his charge in writing, and answered it in the same manner, requesting Elizabeth to content herself with the government of her own kingdom, and not to interfere in the concerns of monarchs as independent as herself. When Tamworth took leave, he refused the passport given him bearing the joint names of the king and queen, out of fear of his imperious mistress, for which Mary ordered him to be apprehended on the road by Lord Home as a vagrant, and detained a couple of days; and on Randolph remonstrating, she informed him that unless he ceased to intrigue with her subjects, she would treat him the same. This bold rebuff to the meddling Queen of England, and the demonstration of affection on the part of the people, confounded the disaffected lords, and their resistance collapsed.
The Queen of Scots, victorious by arms over her enemies, determined to call together a Parliament, and there to procure the forfeiture of Murray and his adherents. This threw the rebel lords into the utmost consternation; for, in the then temper of the nation at large, the measure would have been passed, and they would have been stripped of their estates and entirely crushed. To prevent this catastrophe no time was lost. It was actively spread amongst the people that Mary, having signed the league, it was the intention, through the Kings of France and Spain, to put down the Reformation in Scotland. It was represented that David Rizzio, a Milanese, who had become Mary's secretary for the French language, was the agent of the league and a pensioner of Rome, and that it was necessary to have him removed. Unfortunately for Rizzio, he had incurred the hatred not only of these Protestant lords, but of Darnley, the queen's husband. That young man had soon displayed a character which could bring nothing but misery to the queen. He was a man of shallow intellect, but of violent passions, and, as is usually the case with such persons, of a will as strong as his judgment was weak. He was ambitious of the chief power, and sullenly resentful because it was denied. Mary, who was of a warm and impulsive temperament, in the ardour of her first affection, had promised Darnley the crown matrimonial, which would have invested him with an equal share of the royal authority; but soon unhappily perceiving that she had lavished her regard on a weak, headstrong, and dissipated person, she refused to comply, fully assured of the mischiefs which such power in his hands would produce. Darnley resented this denial violently. He reproached the queen with her insincerity in most intemperate language; treated her in public with scandalous disrespect; abandoned her society for the lowest and worst company, and threw himself into the hands of his enemies, who soon made him their tool. They persuaded him that Rizzio, who, in his quarrels with the queen, always took her part, and who, as the keeper of the privy purse, was obliged to resist his extravagant demands upon it, was not only the enemy of the nation, the spy and paid agent of foreign princes, but was the queen's paramour, and the author of the resolve to keep him out of all real power. The scheme took the effect that was desired. Darnley became jealous and furious for revenge. His father, the Earl of Lennox, joined him in his suspicions, and it was resolved to put Rizzio out of the way.
Darnley, in his blind fury, sent for Lord Ruthven, imploring him to come to him on a matter of life and death. Ruthven was confined to his bed by a severe illness, yet he consented to engage in the conspiracy for the murder of Rizzio, on condition that Darnley should engage to prevent the meeting of Parliament, and to procure the return of Murray and the rebel chiefs. Darnley was in a mood ready to grant anything for the gratification of his resentment against Rizzio; he agreed to everything; a league was entered into, a new covenant sworn, the objects of which were the murder of Rizzio, the prevention of the assembling of Parliament, and the return of Murray and his adherents. Randolph, the English ambassador, now banished from Scotland for his traitorous collusion with the insurgents, yet had gone no farther than Berwick, where he was made fully acquainted with the plot, and communicated it immediately to Leicester in a letter, dated February 13th, 1566, which yet remains. He assured him that the murder of Rizzio would be accomplished within ten days; that the crown would be torn from Mary's dishonoured head, and that matters of a still darker nature were meditated against her person which he dared not yet allude to.
Mary was not without some warnings of what was being prepared, but she could not be made sensible of her danger, neither could Rizzio; though Damiot, an astrologer, whom he was in the habit of consulting, bade him beware of the bastard. The obscurity attending all such oracles led Rizzio to believe that Damiot alluded to Murray, and Rizzio laughed at any danger from him, a banished man; but we shall see that he received his first wound from another bastard, George Douglas, the natural son of the Earl of Angus.
On the 3rd of March Parliament was opened, and a statute of treason and of forfeiture against Murray and his accomplices was immediately introduced on the Thursday, which was to be passed on the following Tuesday. But on the Saturday evening, the queen, sitting at supper in a small closet adjoining her chamber, attended by her natural sister the Countess of Argyll, the Commendator of Holyrood, Beaton Master of the Household, Arthur Erskine captain of the guard, and her secretary Rizzio, was surprised by the apparition of Darnley suddenly putting aside the arras which concealed the door, and standing for a moment gloomily surveying the group. Behind him came a still more startling figure; it was that of Ruthven, in complete armour, just come from his sick bed, and with a face pale and ghastly as that of a ghost. Mary, who was seven months gone with child, started up at this terrible sight, and commanded Ruthven to be gone; but at this moment Darnley put his arm round her waist as if to detain her, and other conspirators entered, one after another, with naked weapons, into the room. Ruthven drew his dagger, and crying that their business was with Rizzio, endeavoured to seize him. But Rizzio, rushing to his mistress, caught the skirt of her robe, and shouted, "Giustizia! giustizia! sauve ma vie—Madame, sauve ma vie!"
Darnley forced himself between the queen and Rizzio, to separate them from one another, and probably the intention was to drag him out of her presence, and dispatch him. But George Douglas, the bastard, in his impetuosity, drove his dagger into the back of Rizzio over the queen's shoulder, and the rest of the conspirators—Morton, Car of Faudonside, and others—dragged him out to the entrance of the presence-chamber, where, in their murderous fury, they stabbed him with fifty-six wounds, with such blind rage that they wounded one another, and left Darnley's dagger sticking in the body as an evidence of his participation in the deed. This done, the hideous Ruthven, exhausted with the excitement, staggered into the presence of the shrieking queen, and, sinking upon a seat, demanded a cup of wine. Mary upbraided him with his brutality; but he coolly assured her that it was all done at the command of her husband and king. At that moment one of her ladies rushed in crying that they had killed Rizzio. "And is it so?" said Mary; "then farewell tears, we must now study revenge."
It was about seven in the evening when this savage murder was perpetrated. The palace was beset by troops under the command of Morton. There was no means of rousing the city, the queen was kept close prisoner in her chamber, whilst the king, assuming the sole authority, issued letters commanding the three Estates to quit the capital within three hours, on pain of treason, whilst Morton with his guards was ordered to allow no one to leave the palace. Notwithstanding this, Huntly, Bothwell, Sir James Balfour, and James Melville, made their escape in the darkness and confusion; and as Melville passed under the queen's window, she suddenly threw up the sash, and entreated him to give the alarm to the city. Her ruffianly guards immediately seized her, and dragged her back, swearing they would cut her to pieces; and Darnley was pushed forward to harangue the people, and assure them that both the queen and himself were safe, and commanding them to retire in peace, which they did.