3. Scalpel.
4. Forceps.
5. Three yards of tape, one inch wide, and lint.
6. A silver wire suture.
7. A basin of cold water.
8. Brandy and Sal Volatile.
Step 1. See that the piston-rod works properly in the syringe, and that the instrument is fit for use; then place it in the basin of cold water with the cannula to lie till wanted.
Step 2. Place the person supplying the blood on a couch or easy chair in the same chamber, but so that he cannot see the recipient, lest he faint and his blood consequently flow feebly. Tie up the arm as for venesection; lay ready the lancet, and direct the assistant, in charge of the supplier of blood, to keep his thumb on the vein when it is opened, that the flow may be checked when blood is not required.
Step 3. Place a tape round the arm of the recipient, above the point for injection, and another below it at a convenient distance, and lay bare a vein (usually the median basilic) for an inch and a half of its course; holding the vein by the forceps, make a slit with the scalpel and introduce the cannula, which is then intrusted to the second assistant. The stylet is withdrawn, and a minute drop of blood escapes through the cannula, showing that the point has been properly introduced into the vein. The assistant replaces the stylet and slackens the ligature, while the surgeon proceeds to fill his syringe.
Step 4. The surgeon, going to the supplier of blood, makes a large opening in the vein with a lancet, or if the first assistant be a surgeon also, he may do this while the chief operator is preparing the vein of the recipient. When the vein is open and the blood flowing freely, the barrel of the syringe is inverted over it and filled with blood; when full, the nozzle is stopped by the plug and the piston attached while the syringe is carried to the recipient.