Aqua Toffana was reputed to possess some very peculiar properties, and, among others, that of causing death at any determinate period, after months, for example, or even years of ill-health (a common supposition attributed to poisons in the Middle Ages). Its alleged effects are graphically described by Behrens as follows: "A certain indescribable change is felt in the whole body, which leads the person to complain to his physician. The physician examines and reflects, but finds no symptoms either external or internal, no vomiting, no inflammation, no fever. In short, he can only advise patience, strict regimen, and laxatives. The malady, however, creeps on, and the physician is again sent for. Still he cannot detect any symptoms of note. Meanwhile the poison takes firmer hold of the system; languor, wearisomeness, and loathing of food continue; the nobler organs gradually become torpid, and the lungs in particular at length begin to suffer. In a word, the malady from the first is incurable; the unhappy victim pines away insensibly even in the hands of the physician, and thus is he brought to a miserable end through months or years, according to his enemy's desire."

Toffana had many imitators, and some time after her death a similar scheme was attempted with a poisonous solution reputedly sold as a cosmetic, called the "Acquetta di Perugia." It is said to have been prepared by killing a hog, disjointing it, strewing the pieces with white arsenic, which was well rubbed in, and finally collecting the juice which dropped from the meat itself. This preparation was supposed to be much stronger and a more powerful poison than arsenic itself, but doubtless had the same fatal effect.

It is a curious fact that most of the notorious poisoners in mediæval times were women, and, indeed, in later years the frail sex seem to have retained a special predilection for this form of crime. In the year 1659, a secret society of women, most of whom were young wives belonging to some of the best and wealthiest families of Rome, was discovered in that city, the sole or chief object of which was to destroy the lives of the husbands of the members. They met at regular intervals at the house of one Hieronyma Spara, a woman reputed to be a witch, who provided her fellow associates and pupils with the required poison, and planned and instructed them how to use it. Operations had been carried on for some time, when the existence of the society was discovered and, says a chronicler, "the hardened old hag passed the ordeal of the rack without confession; but another woman divulged the secrets of the sisterhood, and La Spara, together with twelve other women implicated, were hanged." Many others who were guilty in a lesser degree were publicly whipped through the streets of the city.

In the seventeenth century the mania for poisoning seems to have spread to France, and great interest was excited by the disclosures which followed the discovery of Exili's conspiracy to poison a number of persons. Madame de Montespan, one of the favourites of Louis XIV, a woman of great beauty, died very suddenly at the age of twenty-six, on June 30, 1672, and it was generally believed she had been poisoned. The rumour seems to have been set on foot by one of her husband's old servants, who professed to know the individual who had administered the fatal dose. "This man," said he, "who was not rich, withdrew immediately afterwards into Normandy, where he bought an estate, on which he lived with grandeur a long time; the poison was powder of diamonds, mixed, instead of sugar, with strawberries."

Voltaire, who believed the whole story to be a myth, states: "The court and city believed the princess had been poisoned with a glass of water of succory, after which she felt terrible pains, and soon after was seized with the agonies of death; but the natural malignity of mankind, and a fondness for extraordinary incidents, were the only inducements to this general persuasion. The glass of water could not be poisoned, since Madame de la Fayette and another person drunk what remained without receiving the least injury from it. The princess had been a long time ill of an abscess, which had formed itself in the liver." For some time the young Chevalier De Lorraine, the favourite of the Duke of Orleans, rested under suspicion, it being openly stated that the motive was to revenge the banishment and imprisonment which his misbehaviour to the princess a short time before had drawn upon him. Public opinion was strengthened in the belief that the princess had met her death through poison, by the fact that just at this time the mania for secret poisoning seemed to spread over France. About this date a German apothecary and alchemist, named Glaser, settled in Paris, together with two Italians, one of whom was called Exili. Their professed object was a research to discover the Philosopher's Stone. Having lost the little they possessed in a very short time in the pursuit of this chimera, they commenced the secret sale of poisons. Through the confessional their nefarious trade became known to the Grand Penitentiary of Paris. This dignitary gave information to the Government, and the two suspected Italians were promptly sent to the Bastille, where one of them died; but Exili, while still in prison, managed to carry on his business, and found ready purchasers for his secrets, and the number of deaths attributed to poison increased to such an extent, that a special court for the investigation of poisoning cases, called "La Chambre Ardente," was formed. A few years later the whole of France was aroused by the confession of the Marquise de Brinvilliers of having poisoned her father, two brothers, and a sister. Her husband, the Marquis de Brinvilliers, invited a friend, one Captain St. Croix, who was an officer in his regiment, to lodge in his house. The too agreeable person of the lady of the house speedily charmed the visitor, and to her credit she endeavoured to inspire her husband with a fear of the consequences; but he obstinately persisted in keeping his young friend in the house with his wife, who was both young and handsome, with the result they soon conceived a passion for each other. The father of the marquise, one Lieutenant Daubrai was greatly incensed on hearing of his daughter's indiscretions, and obtaining a lettre de cachet had the captain sent to the Bastille. Here St. Croix was placed in the same cell as Exili, and the latter soon instructed him how he might easily revenge himself. The marquise, who found means of visiting her lover, was informed how to obtain the poison, and at once commenced operations on those members of her family who were most incensed against her, with the result, that first her father, then her brothers and sister fell victims to her revenge. Suspicion resting on her, she fled into Belgium, and was arrested at Liège. A full confession of her crimes, written by her own hand, was found upon her.

She was eventually beheaded, and burnt near Notre Dame in July, 1676. St. Croix is said to have accidentally succumbed to the effects of poisonous fumes in his own laboratory. The authorities on examining his effects, as he left no family, came across a small box to which a paper was attached, which contained a request that after his death "it might be delivered to the Marquise de Brinvilliers, who resides in Rue Neuve St. Paul." This paper was signed and dated by St. Croix on May 25, 1672. On the box being opened, it was found to contain a large collection of various poisons, including corrosive sublimate, antimony, and opium. When the marquise heard of the death of her lover, she at once made every effort to obtain the box by bribing the officers of justice, but failed. La Chaussée, the servant of St. Croix, laid claim to the property, but was arrested as an accomplice and imprisoned. On confessing many serious crimes he was broken alive on the wheel in 1673. Evidence was brought to prove at the trial of De Brinvilliers, that both she and St. Croix were secretly combined with other persons accused of similar crimes. Some distinguished people were implicated, including Pennautier, the receiver-general of the clergy, who was afterwards accused of practising her secrets. One crime seemed to bring another to light, and two persons, named La Voisin and La Vigoreux, a priest named Le Sage, and several others, were next haled before the tribunal, and charged with trading with the secrets of Exili and inciting people with weak minds to the crime of poisoning. It was alleged that through their instrumentality a large number of married women had hastened the decease of their husbands.

The Chambre Ardente, or Burning Court, as it was commonly called, was established at the Arsenal, near the Bastille, and was rarely idle. Persons of the highest rank were cited to appear before it; among others, two nieces of Cardinal Mazarin, the Duchess of Bouillon, and the Countess de Soissons, mother of Prince Eugène. The Countess de Soissons had to retire to Brussels.

The Marshal de Luxemburg was the next sensational arrest. He was carried to the Bastille and submitted to a long examination, after which he was allowed to remain fourteen months in prison. La Voisin and his accomplices were eventually condemned and burnt at the stake, which seemed to put a check on this series of abominable crimes which spread throughout France from 1670 to 1680.

Maria Louisa, daughter of Louis XIV, who married Charles II, King of Spain, is said to have died from the effects of poison in 1689. Voltaire states: "It was undoubtedly believed that the Austrian Ministers of Charles II would get rid of her, because she loved her country and might prevent the king, her husband, from declaring for the allies against France; they even sent her from Versailles what they believed to be a counter-poison." This did not arrive until after her death. In the memoirs of the Marquis de Dangeau, he says: "The king announced the death of his daughter at supper in these words—'The Queen of Spain is dead, poisoned by eating of an eel pye; and the Countess de Pernits and the Cameras, Zapeita, and Nina, who eat of it after her, are also dead of the same poison.'" It is more than probable the unfortunate queen and her ladies succumbed to some putrefactive poison in the fish itself, and were not killed by intent. Nothing was known of animal poisons in those days, and such was the state of the public mind that nearly every sudden death was at once attributed to poison.

The close of the reign of Louis XIV was marked by the sudden deaths of no less than six members of the royal family in close succession. The public sorrow and excitement were great, and rumours and suspicions of poisoning were revived with fury unexampled. The prince had a laboratory, and among other arts studied chemistry. This was considered by the ignorant to be sufficient proof, and the public outcry became terrible. On a visit of the Marquis de Canellae, the prince was found extended on the floor shedding tears, and distracted with despair. His chemist and fellow worker, Homberg, ran to surrender himself at the Bastille, but they refused to receive him without orders. The prince was so beside himself on hearing the public outcry and suspicions that he demanded to be put in prison so that his innocence might be cleared by judicial forms. The lettre de cachet was actually made out, but not signed. The marquis alone kept his head, and prevailed upon the prince's mother to oppose the lettre de cachet. "The monarch who granted it, and his nephew who demanded it, were both equally wretched," says the historian.