(5) Small bits of a spinach leaf caused in 1 hr. 20 m. increased secretion; and after 3 hrs. 20 m. plain incurvation of the margin. The margin was well inflected after 9 hrs. 15 m., but after 24 hrs. was almost fully re-expanded. The glands in contact with the spinach became dry in 72 hrs. Bits of albumen had been placed the day before on the opposite margin of this same leaf, as well as on that of a leaf with cabbage seeds, and these margins remained closely inflected for 72 hrs., showing how much more enduring is the effect of albumen than of spinach leaves or cabbage seeds .
(6) A row of small fragments of glass was laid along one margin of a leaf; no effect was produced in 2 hrs. 10 m., but after 3 hrs. 25 m. there seemed to be a trace of inflection, and this was distinct, though not strongly marked, after 6 hrs. The glands in contact with the fragments now secreted more freely than before; so that they appear to be more easily excited by the pressure of inorganic objects than are the glands of Pinguicula vulgaris. The above slight inflection of the margin had not increased after 24 hrs., and the glands were now beginning to dry. The surface of a leaf, near the midrib and towards the base, was rubbed and scratched for some time, but no movement ensued. The long hairs which are situated here were treated in the same manner, with no effect. This latter trial was made because I thought that the hairs might perhaps be sensitive to a touch, like the filaments of Dionaea. [page 394]
(7) The flower-peduncles, sepals and petals, bear glands in general appearance like those on the leaves. A piece of a flower-peduncle was therefore left for 1 hr. in a solution of one part of carbonate of ammonia to 437 of water, and this caused the glands to change from bright pink to a dull purple colour; but their contents exhibited no distinct aggregation. After 8 hrs. 30 m. they became colourless. Two minute cubes of albumen were placed on the glands of a flower-peduncle, and another cube on the glands of a sepal; but they were not excited to increased secretion, and the albumen after two days was not in the least softened. Hence these glands apparently differ greatly in function from those on the leaves.]
From the foregoing observations on Pinguicula lusitanica we see that the naturally much incurved margins of the leaves are excited to curve still farther inwards by contact with organic and inorganic bodies; that albumen, cabbage seeds, bits of spinach leaves, and fragments of glass, cause the glands to secrete more freely;—that albumen is dissolved by the secretion, and cabbage seeds killed by it;—and lastly that matter is absorbed by the glands from the insects which are caught in large numbers by the viscid secretion. The glands on the flower-peduncles seem to have no such power. This species differs from Pinguicula vulgarisand grandiflora in the margins of the leaves, when excited by organic bodies, being inflected to a greater degree, and in the inflection lasting for a longer time. The glands, also, seem to be more easily excited to increased secretion by bodies not yielding soluble nitrogenous matter. In other respects, as far as my observations serve, all three species agree in their functional powers. [page 395]
CHAPTER XVII.
UTRICULARIA.
Utricularia neglecta—Structure of the bladder—The uses of the several parts—Number of imprisoned animals—Manner of capture—The bladders cannot digest animal matter, but absorb the products of its decay—Experiments on the absorption of certain fluids by the quadrifid processes—Absorption by the glands—Summary of the observation on absorption— Development of the bladders—Utricularia vulgaris—Utricularia minor—Utricularia clandestina.
I was led to investigate the habits and structure of the species of this genus partly from their belonging to the same natural family as Pinguicula, but more especially by Mr. Holland’s statement, that “water insects are often found imprisoned in the bladders,” which he suspects “are destined for the plant to feed on.”* The plants which I first received as Utricularia vulgaris from the New Forest in Hampshire and from Cornwall, and which I have chiefly worked on, have been determined by Dr. Hooker to be a very rare British species, the Utricularia neglecta of Lehm.** I subsequently received the true Utricularia vulgaris from Yorkshire. Since drawing up the following description from my own observations and those of my son, Francis Darwin, an important memoir by Prof. Cohn
* The ‘Quart. Mag. of the High Wycombe Nat. Hist. Soc.’ July 1868, p. 5. Delpino (‘Ult. Osservaz. sulla Dicogamia,’ &c. 1868-1869, p. 16) also quotes Crouan as having found (1858) crustaceans within the bladders of Utricularia vulgaris.
** I am much indebted to the Rev. H.M. Wilkinson, of Bistern, for having sent me several fine lots of this species from the New Forest. Mr. Ralfs was also so kind as to send me living plants of the same species from near Penzance in Cornwall. [page 396]