New York Journal of Pharmacy, Volume 1 (of 3), 1852 / Published by Authority of the College of Pharmacy of the City of New York. - College of Pharmacy of the City of New York

II. 0.2415 grm. aloine gave 0.5250 carbonic acid and 0.126 water. {180}

	Hydrated aloine.		Found numbers.
	Calculated numbers.		I.	II.
34 C	2550.0	59.47	59.39	59.24
19 H	237.5	5.54	5.97	5.79
15 O	1500.0	35.09	34.64	34.97
	4287.5	100.00	100.00	100.00

The formula derivable from these analyses is C₃₄ H₁₉ O₁₅, which, as we shall presently see, is = C₃₄ H₁₈ O₁₄ +HO, or aloine with one equivalent of water.

The aloine which had been dried in vacuo was next heated in the water-bath for five or six hours, and was also subjected to analysis.

I. 0.251 grm. aloine dried at 212° F. gave 0.550 carbonic acid and 0.128 water.

II. 0.2535 grm. aloine dried at 212° F. gave 0.564 carbonic acid and 0.129 water.

III. 0.234 grm. aloine dried at 212° F. gave 0.521 carbonic acid and 0.114 water.

	Calculated numbers.		I.	II.	III.
34 C	2550	61.07	60.51	60.67	60.72
18 H	225	5.39	5.66	5.65	5.42
14 O	1400	33.54	33.83	33.68	33.86
	4175	100.00	100.00	100.00	100.00

The aloine employed in these analyses was prepared at three different times. These results give C₃₄ H₁₈ O₁₄ as the formula of anhydrous aloine, that dried in vacuo being a hydrate with one equivalent of water.

When the aloine was allowed to remain in the water-bath for more than six hours, it continued slowly to lose weight, apparently owing to its undergoing partial decomposition by the formation of a brownish resin. The loss of weight gradually continued for a week or more, but became very rapid when the aloine was heated to 302° F., when it melted, forming a dark brownish mass, which when cooled became as hard and brittle {181} as colophonium. It still, however, contained a good deal of unaltered aloine, as I ascertained by crystallizing it out with hot spirits and analyzing it. Much of the aloine, however, had been changed, most probably by oxidation, into a dark brown uncrystallizable resin.