[E] Hist. Anim. lib. vi. cap. 2.

[F] Hist. Anim. lib. vi. cap. 4.

Harvey attacks Aristotle for saying that the yolk ascends towards the small end of the egg, and concludes, that he had not seen any thing himself, but had apparently received his information from some good observer of Nature. Harvey was wrong in thus reproaching Aristotle, and in asserting that the yolk always ascends towards the broad end of the egg, for that depends on the position of the egg during the time of incubation, for the yolk always ascends to the uppermost part, as being lighter than the white, whether it be to the broad or the small end. William Langley, a physician at Dordrecht, who made observations on the hatching of eggs, in 1655, twenty years before Harvey, was the first who made this remark.[G]

[G] See Wm. Langley Observ. edæ a justo Schradero, Amst. 1674.

But to return to the passage we have quoted. By that we see that the crystalline liquor, the animated speck, the two circles, the two blood vessels, &c. are described by Aristotle precisely as Harvey had seen them. This anatomist also pretends that the animated speck is the heart, that this heart is formed the first, and that the viscera and other parts are joined afterwards. All this has been spoken of by Aristotle, and seen by Harvey, and nevertheless it is not conformable to truth. To be assured of this we need only repeat the same experiments on eggs, or only read with attention those of Malpighius,[H] which were made about 40 years after those of Harvey.

[H] Malpighii pullus in ovo.

This excellent observer of Nature examined, with attention, the cicatrice, which is the essential part of the egg; he found it was large in all impregnated eggs, and small in those which were not impregnated; and he discovered in eggs which had never been sat upon, that the white speck, spoken of by Harvey as the first which becomes animated, is a small pouch or ball, which swims in a liquor inclosed by the first circle, and in the middle of this ball he observed the embryo. The membrane of this small pouch, which is the amnios, being very thin and transparent, permitted him easily to see the fœtus it surrounded. Malpighius, with reason, concludes, from this first observation, that the fœtus exists in the egg before incubation, and that its first outlines are then very strong. It is not necessary to point out how opposite this experiment is to the opinion of Harvey, for he saw nothing of a form for the two first days of incubation, and it was the third day before the sign of the fœtus appears, which is the animated speck: whereas according to Malpighius, the outlines of the fœtus exist in the egg before incubation has commenced.

After being assured of this important matter, Malpighius examined, with like attention, the cicatrice of unimpregnated eggs, which, as we have observed, is smaller than those which have been impregnated; it has often irregular circumscriptions, and sometimes differs in different eggs. Near its centre, instead of the ball that encloses the fœtus, there is a globular mole, which does not contain any thing organized, and which being opened does not present any thing formed or arranged, but only some appendages filled with a thick but transparent fluid; and this unshapen mass is surrounded with many concentric circles.

After six hours incubation the cicatrice is considerably dilated, and the ball formed by the amnios is easily discovered; this ball is filled with a liquor, in the middle of which the head of the chicken and back-bone are distinctly seen. In about six hours more the little animal is seen more distinctly; in another six hours the head is grown larger, and the spine lengthened; and at the end of twenty-four hours the neck begins to lengthen, the vertebræ of the back appears of a white colour, and the head to turn to one side. The vertebræ are disposed on each side of the spine, like small globules; and almost at the same time the small wings begin to shoot, and the head, neck, and breast are lengthened. After thirty hours nothing new appears, but every part of the little animal is considerably increased, especially the amnios. Around this membrane the umbilical vessels are seen of a darkish colour. At the end of thirty-eight hours, the chicken being grown much larger, its head is large, and in which are distinguished three vessels surrounded with membranes, which also cover the back bone, through which the vertebræ are still seen. In forty hours, continues Malpighius, it was wonderful to see the chicken alive, floating in the liquor; the back bone was increased, the head was turned on one side, the vesicles of the brain were less apparent, the first outlines of the eyes appeared, the heart beat, and the circulation of the blood was begun. Malpighius then gives the description of the vessels and course of the blood, and reasonably supposes that, though the heart does not beat before thirty-eight or forty hours incubation, it still existed before that time, like the other parts of the chicken; but on examining the heart in a dark room, he discovered not the least glimpse of light to proceed from it, as Harvey insinuates.

At the end of two days the chicken is seen floating in the liquor of the amnios; in which the head, composed of vesicles, is turned on one side; the back bone and vertebræ are lengthened; the heart, which then hung out of the breast, beat three times; for the fluid it contains is impelled into the ventricles of the heart, from thence into the arteries, and afterwards into the umbilical vessels. He remarks, that having separated the chick from the white of the egg, the motion of the heart still continued for a whole day. After two days and fourteen hours, or sixty-two hours of incubation, the chicken, although grown stronger, remained with its head bent downwards in the liquor, contained by the amnios; the veins and arteries were seen among the vessels of the brain; the lineaments of the eyes, and the spinal marrow, also appear extending the length of the vertebræ.