Method of staining:
1. Make films and air dry.
2. Cover dry film preparation with the methyl-alcohol stain for one minute (to fix).
3. Add water to the stain on the cover-glass or slide, drop by drop, until a yellow metallic scum begins to form. It is advisable to add the drops of water rapidly in order to eliminate precipitates on the stained film. Practically, we may add 1 drop of water for every drop of stain used.
4. Wash thoroughly in water until the film has a pinkish tint.
5. Dry with filter-paper and mount.
Red cells are stained orange to pink; nuclei, shades of violet; eosinophile granules, red; neutrophile granules, yellow to lilac; blood platelets, purplish; malarial parasites, blue; chromatin, metallic-red to rose-pink.
Giemsa’s Modification of the Romanowsky Method.—This is one of the most perfect of the modifications. The objection is that greater time in staining films is required than with the Wright or Leishman method and the stain is very expensive.
Take of Azur II eosin 0.3 gram. Azur II 0.08 gram.