Fifth lobe removed: 17 pulsations plus 15 equals 32 to the minute.
Sixth lobe removed: 17 in first half-minute plus 4 in the second half-minute gives 21 pulsations for the minute.
Seventh lobe removed: 17 plus 9, or 26 per minute.
In all these instances the rhythm in the second half of the first minute was irregular and intermittent.
Seventeen and then seven pulsations were provoked after the animal had become quiescent, or nearly so, by merely handling it.
45. Eighth oral lobe was removed and pulsations stopped. The next day the animal was in good condition. The pulsations counted in the evening were 12, 14, 14, 11, per minute. The rhythm was not regular; there was a tendency to groups of twos, threes, or more, but no prolonged intervals of rest were observed. When placed into fresh sea-water, the pulsations were fourteen to the half-minute or twenty-six to the minute; seventeen to the half-minute, and thirty-three to the minute were also counted. This specimen gave spontaneous contractions during two weeks, after which it was thrown out, the aboral end being eaten through and little or no regeneration having taken place.
46. Two more were operated upon: A. Its rhythm was 18, 14, 17. Its entire margin was cut off. The separate pieces of the margin pulsated, 6, 7, 4, 6, 7, 9. The animal seemed paralyzed by the operation; it responded by a contraction now and then to stimulation but gave no spontaneous pulsations. B. Its rhythm was 17, 15, 12, 12. All its oral arms were removed. Its rhythm was only raised to seventeen and not perfect. In twenty-five minutes it had fallen to eleven, in four hours to ten pulsations [per minute].
May 22nd. A and B are living as also the pieces of the margin of A; all are giving spontaneous pulsations now and then at comparatively long intervals—even A, with its margin removed.
May 26th. Everything is still living. The one with the margin cut (A) counted sixteen and nineteen pulsations per minute, though this was not kept up all the time.