In 1509 the Venetians, taking advantage of Maximilian's vacillation, recaptured Padua. The surrounding population and peasantry immediately rose in favour of the republic, which recovered the town and fortress of Legnago. Padua's capitulation did not prevent Louis XII. from recrossing the Alps after he had concluded a new treaty with Pope Julius II. at Biagrassa, in which they mutually promised to help each other. Maximilian now decided to crush the republic by a decisive blow in laying siege to the capital. But although Louis seemed to agree with this plan, the Pope disapproved, and Ferdinand formally opposed it.

The emperor finding it impossible to lay siege to Venice without help from his allies, prepared to retake Padua; but after sixteen days of firm resistance from the Venetian garrison, he withdrew to Limini, on the way to Treviso. From there he went to Vicenza and Verona, bitterly complaining of the treatment he had received from the Pope and the King of France, because the former had consented to receive the Venetian ambassadors, and the latter had caused the loss of Padua through his delay in sending help. Having failed to retake Legnago, Maximilian seemed inclined to make a truce with Venice, but the republic turned a deaf ear to his advances, and he returned to Trent discontented with himself and his allies.

Julius II.'s changeable policy increased the dissensions which undermined the League. In spite of remonstrances from Maximilian's and Louis' envoys, Julius wished to receive the Venetian ambassadors and pardon the republic. He was secretly encouraged in this by the King of Aragon and openly by the Archbishop of York, representing Henry VIII. Julius thought he could save the republic by overthrowing the French rule in Italy, and for this reason he made friends with England, and encouraged the Swiss in their discontent with France.

In a long letter to his daughter Queen Katharine, written on November 18th, 1509, King Ferdinand says 'that he has touched on the subject of the preservation of Venice in his negotiations with the King of France, but very cautiously and without discovering his plans, his intention being to keep his negotiations secret until he has won over Maximilian. He tells Katharine that a short time ago Madame Margaret sent her secretary to him. The secretary spoke about the alliance, but he (Ferdinand) intends to make a further communication to Madame Margaret, who is the person who has the greatest influence with her father, and she would think herself honoured if so important a business as the conclusion of the alliance were intrusted to her hands. He begs Katharine to see that the English envoy who is sent to Madame Margaret is an honest, intelligent, and discreet man,' and adds, 'he must go alone and not be accompanied by any other person, and it is necessary that he should be able to speak and express himself well on the subject he has in hand.'[39]

In the following December Miguel Perez Almazan, King Ferdinand's First Secretary of State, wrote to Margaret's secretary to inform him that news had arrived that the King of France intended seizing the cities of Verona and Vicenza; and that he was also making preparations to besiege Venice on every side. 'If he should carry out his designs he would probably become master of Italy and perhaps of Christendom, unless the emperor and King Ferdinand take prompt means to stop him, which they ought to do for the sake of their common grandson, Prince Charles.' Almazan goes on to state that Henry VIII. had sent a letter to King Ferdinand, in which he expressed a wish to enter into a close alliance with Spain and the emperor. If such an alliance were concluded the King of France would be kept from injuring the allies.

'This letter,' he says, 'is sent in order that the secretary may use his influence with Madame Margaret, and induce her to help forward the alliance between the emperor, Spain, and England; a task which is certainly not difficult for her, and the execution of which would secure her lasting fame.'[40]

Whilst Maximilian was trying to extract a subsidy for the continuance of the Venetian war from the Diet at Augsburg, Julius II. was maturing his plans. When the Venetian ambassadors accepted his proposed treaty (24th of February 1510), he took them back into favour, and solemnly gave them absolution.

Subjects and vassals of the Church were bidden to help the republic, and Julius openly quarrelled with the Duke of Ferrara, who wished to remain faithful to the League of Cambray. He urged Henry VIII. to declare war against France, and King Ferdinand secretly did the same. As the Pope observed, the object of the League of Cambray ceased to exist.

At this crisis Louis XII. lost his faithful friend and able Minister, Cardinal d'Amboise. He was succeeded by Florimond Robertet, who had none of his predecessor's great qualities. The Cardinal died at Lyons on the 26th of May 1510. André de Burgo was then Austrian ambassador at the French Court, and writing to inform Margaret of the Cardinal's death, he says, 'I assure you your House has suffered a great loss.'

Encouraged by the death of Georges d'Amboise, the Pope continued to make preparations, and declared that God had chosen him to be the Liberator of Italy. In spite of his age and infirmities he was present at the siege of Mirandola, in January 1511, and entered the town by a breach.