2. 96% alcohol, in proportion to twice the amount of cerebrospinal fluid, is added drop by drop and well mixed.
3. Centrifuge the mixture for one hour at high speed in a glass tube with conical end. (An ordinary electric urinary centrifuge apparatus can be employed, the tube to be well stoppered to prevent evaporation.)
4. The supernatant fluid is poured off, leaving a small coagulum in the bottom of the tube.
5. Add absolute alcohol—alcohol and ether—ether, each separately for one hour, to dehydrate and harden coagulum.
6. The coagulum can now be gently loosened from the bottom of the tube by a long needle. The tube is then inverted, and the coagulum allowed to fall into the hand by a quick tap on the end of the tube. Care must be taken not to squeeze or handle the coagulum. The hand is placed over a small homeopathic vial, containing thin celloidin, and the coagulum allowed to drop into the celloidin, where it remains over night (twelve hours usually).
7. Coagulum is placed in thick celloidin which is allowed to evaporate slowly. It is then mounted on blocks and sections cut 14µ in thickness.
8. The sections are stained and mounted according to the following procedure:
(a) Remove celloidin by absolute alcohol and ether.
(b) 80% alcohol.
(c) Water.