This label is interpreted as follows:
Dry Tb., which in the records of the Marburg Institute bears the laboratory number IV, contain on the day of delivery, i.e., on VII-17-02, twenty immunizing units, and retain this strength until VIII-17-02.
For the inoculation the virus is uniformly mixed with 1% salt solution which has previously been boiled and cooled. The procedure is as follows:
The entire contents of the tube are placed in a small mortar and crushed with the pestle. Then 2 to 3 c.c. salt solution are added and the whole rubbed into a uniform mixture or emulsion, after which it is poured into a graduated cylinder holding 50 c.c. A little more salt solution is then added to the mortar, and thus the remaining particles of virus are added to the previous mixture. The fluid in the graduate is then made up to 30 c.c. with salt solution, and then poured into a sterile wide-mouthed bottle holding 100 c.c. Any remaining emulsion is then washed out of the graduate with an additional 10 c.c. salt solution and added to the 30 c.c. in the bottle. The bottle thus contains virus ready to inoculate, and each 2 c.c. will be the dose for the first inoculation of a calf, provided that the tube originally contained 20 I. E. The dose for a second inoculation would then be contained in 10 c.c. of this fluid.
VII. Instrument Case.—In order to carry out the inoculations in agricultural practice, the instrument case constructed by W. Holzhauer, Marburg, is to be recommended. It contains the following:
(a) Two bottles for the virus, each 100 c.c., made of colored glass.
(b) One bottle lysol.
(c) One bottle alcohol.
(d) One Erlenmeyer flask (for 1% salt solution).
(e) One mortar and pestle.