The nerve itself is still, as in the last case, composed of cells which are larger and more elongated than either the cells of the root or the ganglion.
The condition of the anterior root at this stage is hardly altered from what it was; it is composed of very small cells, which with hæmatoxylin stain more deeply than any other cell of the section. A figure of it is given in I II.
Horizontal longitudinal sections of this stage are both easy to make and very instructive. On Pl. 23, fig. K I is represented a horizontal section through a plane near the dorsal surface of the spinal cord: each posterior root is seen in this section to lie nearly opposite the anterior extremity of a muscle-plate.
In a more ventral plane (fig. K II) this relation is altered, and the posterior roots lie opposite the hinder parts of the muscle-plates.
The nerves themselves are invested by the hyaline membrane spoken of above; and surrounding this again there is present a delicate mesoblastic investment of spindle-shaped cells.
Longitudinal sections also throw light upon the constitution of the anterior nerve roots (vide fig. K II, ar). In the two segments on the left-hand side in this figure the anterior roots are cut through as they are proceeding, in a more or less horizontal course, from the spinal cord to the muscle-plates.
Where the section (which is not quite horizontal) passes through the plane of the notochord, as on the right-hand side, the anterior roots are cut transversely. Each root, in fact, changes its direction, and takes a downward course.
The anterior roots are situated nearly opposite the middle of the muscle-plates: their section is much smaller than that of the posterior roots, and with hæmatoxylin they stain more deeply than any of the other cells in the preparation.
The anterior roots, so far as I have been able to observe, do not at this stage unite with the posterior; but on this point I do not speak with any confidence.
The period now arrived at forms a convenient break in the development of the spinal nerves; and I hope to treat the remainder of the subject, especially the changes in the ganglion, the development of the ganglion-cells, and of the nerve-fibres, in a subsequent paper.