Figs. I I, I II. Two sections of a Pristiurus-embryo somewhat older than H. Camera. (Zeiss CC ocul. 1.)

The connective-tissue cells are omitted.

Figs. I a, I b, I c. Three isolated cells from the ganglion of one of the posterior roots of the same embryo.

Figs. K I, K II. Two horizontal longitudinal sections through an embryo in which the external gills have just appeared. K I is the most dorsal of the two sections. Camera. (Zeiss CC ocul. 1.)

The sections shew the relative positions of the anterior and posterior roots at different levels.

pr. posterior nerve-rudiment. ar. anterior nerve-rudiment. sp.c. spinal cord. n.c. neural canal. mp. muscle-plate. mp´. first-formed muscles.

Fig. L. Longitudinal and vertical section through the trunk of a Scyllium-embryo after the external gills have attained their full development. Camera. (Zeiss CC ocul. 1.)

The embryo was hardened in a mixture of chromic acid and osmic acid.

The section shews the commissures which dorsally unite the posterior roots, and also the junction of the anterior and posterior roots. The commissures are unfortunately not represented in the figure with great accuracy; their outlines are in nature perfectly regular, and not, as in the figure, notched at the junctions of the cells composing them. Their cells are apparently more or less completely fused, and certainly not nearly so clearly marked as in the figure. The commissures stain very deeply with the mixture of osmic and chromic acid, and form one of the most conspicuous features in successful longitudinal sections of embryos so hardened. In sections hardened with chromic acid only they cannot be seen with the same facility.

sp.c. spinal cord. gr. grey matter. w. white matter. ar. anterior root. pr. posterior root. x. commissure uniting the posterior roots.