Instead of being but indistinctly separated from the surrounding yolk, the blastoderm has now very clearly defined limits.
This is an especially marked feature of preparations made with osmic acid. In these there may frequently be seen a deeply stained doubly contoured line, which forms the limit of the yolk, where it surrounds the germinal disc. Lines of this kind are often to be seen on the surface of the yolk, or even of the blastoderm, but are probably to be regarded as products of reagents, rather than as organised structures. The outline of the germinal disc is well rounded, though it is occasionally broken, from the presence of a larger cell in the act of being formed from the yolk.
It is not probable that any great importance is to be attached to the comparative distinctness of the outline of the germinal disc at this stage, which is in a great measure due to a cessation in the formation of fresh cells in the surrounding yolk, and in part to the small and comparatively uniform size of the cells of the germinal disc.
The formation of fresh cells from the yolk nearly comes to an end during this period, but it still continues on a small scale.
The number of the nuclei around the germinal disc has increased.
Another feature of interest which first becomes apparent during this stage is the asymmetry of the germinal disc. If a section were made through the germinal disc, as it lay in situ in the egg capsule, parallel or nearly so to the long axis of the capsule, one end of the section would be found to be much thicker than the other. There would in fact be a far larger collection of cells at one extremity of the germinal disc than at the other. The end at which this collection of cells is formed points towards the end of the egg capsule opposite to that near which the yolk is situated. This collection of cells is the first trace of the embryo; and with its appearance the segmentation may be supposed to terminate.
The section I have represented, though not quite parallel to the long axis of the egg, is sufficiently nearly so to shew the greater mass of cells at the embryonic end of the germinal disc.
This very early appearance of a distinction in the germinal disc between the extremity at which the embryo appears and the non-embryonic part of the disc, besides its inherent interest, has a further importance from the fact that in Osseous Fishes a similar occurrence takes place. Oellacher[98] and Götte[99] both agree as to the very early period at which a thickening of one extremity of the blastoderm in Osseous Fishes is formed, which serves to indicate the position at which the embryo will appear. There are many details of development in which Osseous Fish and Elasmobranchii agree, which, although if taken individually are without any great importance, yet serve to shew how long even insignificant features in development may be retained.
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The segmentation of the Elasmobranch egg presents in most of its features great regularity, and exhibits in its mode of occurrence the closest resemblance to that in other meroblastic vertebrate ova.