Third day after impregnation.—In this stage the embryo is about 3.5 millims. in length, and has a somewhat dumb-bell shaped outline (Plate 34, fig. 5). It consists of (1) an outer area (p.z) with some resemblance to the area pellucida of the Avian embryo, forming the parietal part of the body; and (2) a central portion consisting of the vertebral and medullary plates and the axial portions of the embryo. In hardened specimens the peripheral part forms a shallow depression surrounding the central part of the embryo.
The central part constitutes a somewhat prominent ridge, the axial part of it being the medullary plate. Along the anterior half of this part a dark line could be observed in all our specimens, which we at first imagined to be caused by a shallow groove. We have, however, failed to find in our sections a groove in this situation except in a single instance (Plate 35, fig. 20, x), and are inclined to attribute the appearance above-mentioned to the presence of somewhat irregular ridges of the outer layer of the epiblast, which have probably been artificially produced in the process of hardening.
The anterior end of the central part is slightly dilated to form the brain (b); and there is present a pair of lateral swellings near the anterior end of the brain which we believe to be the commencing optic vesicles. We could not trace any other clear indications of the differentiation of the brain into distinct lobes.
At the hinder end of the central part of the embryo a very distinct dilatation may also be observed, which is probably homologous with the tail swelling of Teleostei. Its structure is more particularly dealt with in the description of our sections of this stage.
After the removal of the egg-membranes described above we find that there remains a delicate membrane closely attached, to the epiblast. This membrane can be isolated in distinct portions, and appears to be too definite to be regarded as an artificial product.
We have been able to prepare several more or less complete series of sections of embryos of this stage (Plate 35, figs. 18-22). These sections present as a whole a most striking resemblance to those of Teleostean embryos at a corresponding stage of development.
Three germinal layers are already fully established. The epiblast (ep.) is formed of the same parts as in Teleostei, viz.:—of an outer epidermic and an inner nervous or mucous stratum. In the parietal region of the embryo these strata are each formed of a single row of cells only. The cells of both strata are somewhat flattened, but those of the epidermic stratum are decidedly the more flattened of the two.
Along the axial line there is placed, as we have stated above, the medullary plate. The epidermic stratum passes over this plate without undergoing any change of character, and the plate is entirely constituted of the nervous stratum of the epidermis.
The medullary plate has, roughly speaking, the form of a solid keel, projecting inwards towards the yolk. There is no trace, at this stage at any rate, of a medullary groove; and as, we shall afterwards shew, the central canal of the cerebro-spinal cord is formed in the middle of the solid keel. The shape of this keel varies according to the region of the body. In the head (Plate 35, fig. 18, m.c.), it is very prominent, and forming, as it does, the major part of the axial tissue of the body, impresses its own shape on the other parts of the head and gives rise to a marked ridge on the surface of the head directed towards the yolk. In the trunk (Plate 35, figs. 19, 20) the keel is much less prominent, but still projects sufficiently to give a convex form to the surface of the body turned towards the yolk.
In the head, and also near the hind end of the trunk, the nervous layer of the epiblast continuous with the keel on each side is considerably thicker than the lateral parts of the layer. The thickening of the nervous layer in the head gives rise to what has been called by Götte[501] “the special sense plate,” owing to its being subsequently concerned in the formation of parts of the organs of special sense. We cannot agree with Götte in regarding it as part of the brain.