How infection occurs is a question to which at present no satisfactory answer can be made. The attempt to introduce embryos of Bilharzia into the common fresh-water animals of Alexandria has hitherto proved fruitless (Looss[[88]]), although there seems little doubt that the comparative immunity of Europeans from the disease is in some way owing to their drinking purer water than the natives. Possibly, as Leuckart suggests, the embryo becomes a sporocyst in man himself, somewhat as Taenia murina is known to develop in the rat without an intermediate host.[[89]] The immense numbers of the parasite in one host would then readily receive an explanation.

A Bilharzia, possibly B. haematobia, was found by Cobbold in the portal vein of Cercopithecus fuliginosus; and B. crassa infests the cattle of Egypt, Sicily, and certain parts of India, but does not produce haematuria.

Of the other Trematodes of man and domestic animals there is not room to speak fully. Distomum pulmonale, which occurs in the lungs of the cat, tiger, and dog, as well as in man, is especially common in Japan, China, Corea, and Formosa. D. sinense and D. rathouisi have been also found in inhabitants of these countries.

Bisexual Trematodes.—Zoologically, Bilharzia is interesting from its bisexual condition. It is not, however, the only bisexual Trematode. In cysts in the branchial chamber of Ray's bream, Brama raii, two worms are found, which are probably the slender male and the swollen female of the same species (Distomum okenii). The only doubt that can arise proceeds from the tendency in all Trematodes for the male organs to ripen before the female organs. Until we certainly know that the swollen egg-bearing form (♀) does not arise from a previously male form (♂), the case is open to suspicion. Since, however, Kölliker[[90]] never found intermediate hermaphrodite conditions, this Distomum may be almost certainly regarded as of distinct sexes. Didymozoon thynni (Monostomum bipartitum), from cysts on the gills of the Tunny (Thynnus), is another case. Two slender worms flattened posteriorly, come together, and the body of one becomes folded to receive that of the other. They fuse completely except for a small lateral opening through which the anterior parts of both worms may freely protrude. The enclosing individual contains a coiled uterus filled with eggs, and is the female, whereas the smaller individual never possesses eggs, and is probably the male.[[91]] Nematobothrium (Fig. 22, A), which occurs also in the Tunny, in the form of two immensely long individuals intricately wound about each other in a cyst, is, however, not bisexual.

Fig. 35.—Distomum okenii Köll. Showing male and female as they occur together in the branchial cavity of Bramaraii (Ray's bream). (From Bronn, after Kölliker.) Nat. size.

Table of Digenetic Trematodes and their Life-Histories.[[92]]

Species.Final host.Host into which the larva enters, and in which Cercariae are eventually formed.Host into which the Cercariae migrate and encyst; eaten by final host.
Diplodiscus (Amphistomum) subclavatus GözeRana, Bufo, TritonSmaller species of Planorbis and CyclasInsect-larvae, Rana, Bufo, but frequently omitted
Distomum advena Duj. (D. migrans Duj.)Sorex araneusNot knownLimax
D. appendiculatum Rud.Clupea alosaNot knownLucullus acuspes, Centropages hamatus (Copepoda)
D. ascidia v. Ben.Species of Bats Limnaea stagnalis Planorbis corneus Ephemera, Perla, Chironomus plumosus
D. atriventre Weinl.Frogs and Toads of N. AmericaPhysa heterostrophaNot known
D. brachysomum Crepl.The Dunlin (Tringa alpina)Not knownAnthura gracilis
D. caudatum v. Linst.Hedgehog (Erinaceus europaeus)Helix hortensis
D. clavigerum Rud. Rana Limnaea ovata Planorbis corneus Not known
D. cygnoides Zed.RanaPisidium, CyclasLimnaea sp. (Cercaria macrocerca Fil.)
D. cylindraceum Zed.RanaLimnaea ovataIlybius fuliginosus
D. dimorphum Dies.Ardea, Ciconia (Brazil)Not knownDifferent species of Fishes
D. echinatum Zed.Cygnus, Anser, AnasSpecies of LimnaeaSpecies of Limnaea, Paludina vivipara
D. endolobum Duj.RanaLimnaea stagnalisL. stagnalis, Gammarus pulex, larvae of Limnophilus rhombicus
D. globiporum Rud.Perca fluviatilisNot knownLimnaea stagnalis, L. ovata, Succinea pfeifferi, S. putris, Physa fontinalis, Planorbis marginatus
D. hepaticum Abild.Sheep, Oxen, Man, etc.Limnaea truncatulaOmitted
D. hystrix Duj.Lophius piscatoriusNot knownMarine Fishes
D. macrostomum Rud.Warblers, Tits, Woodpeckers, etc.Succinea putrisOmitted
D. militare v. Ben.Common SnipePaludina viviparaP. vivipara
D. nodulosum Zed.Perca fluviatilisBithynia tentaculataCyprinus, Acerina cernua
D. ovocaudatum Vulp.Rana esculentaSpecies of PlanorbisProbably omitted. (Cercaria known as C. cystophora Wag.)
D. retusum Duj.RanaLimnaea stagnalisL. stagnalis, larvae of Phryganeidae
D. squamula Dies.PolecatUnknownRana temporaria
D. signatum Duj.Tropidonotus natrixUnknownRana
D. trigonocephalum Rud.Badger, PolecatPaludina viviparaUnknown
Gasterostomum sp.Dogfish, RaysOstrea edulis, Cardium rusticum, C. eduleBelone vulgaris
G. fimbriatum v. Sieb.Perca, EsoxUnio, Anodonta (Cercaria known as Bucephalus polymorphus)Leuciscus erythrophthalmus
G. gracilescens Rud.Lophius piscatoriusUnknownSpecies of Gadus (e.g. G. aeglefinus), Molva, Lophius
Monostomum flavum Mehl.AnasPlanorbis corneusOmitted

Classification of Trematodes.—We have seen (p. [63]) that it is hardly possible to carry out fully the division of Trematodes into Monogenea and Digenea. Nevertheless, pending further investigation on the doubtful points, this classification may still be used. Monticelli[[93]] has proposed the main divisions of a new classification, which has been also adopted by Braun, and is based on the nature of the suckers. These divisions are indicated below in brackets.

A. Monogenea v. Ben. (Heterocotylea Mont.).
1. Fam. Temnocephalidae Hasw.
Gen. Temnocephala Hasw.
2. Fam. Tristomatidae Tschbg.
Sub-Fam. 1. Tristomatinae Mont.
Gen. Tristomum, Nitzschia, Epibdella, Trochopus, Acanthocotyle, Phyllonella, Placunella, Encotylabe.
Sub-Fam. 2. Monocotylinae Tschbg.
Gen. Pseudocotyle, Calicotyle, Monocotyle.
Sub-Fam. 3. Udonellinae v. Ben.-Hesse.
Gen. Udonella, Echinella, Pteronella.
3. Fam. Polystomatidae Tschbg.
Sub-Fam. 4. Octocotylinae v. Ben.-Hesse.
Gen. Octobothrium, Pleurocotyle, Diplozoon, Anthocotyle, Vallisnia, Phyllocotyle, Hexacotyle, Platycotyle, Plectanocotyle, Diclidophora.
Sub-Fam. 5. Polystomatinae v. Ben.
Gen. Polystomum, Onchocotyle, Erpocotyle, Diplobothrium, Sphyranura.
Sub-Fam. 6. Microcotylinae Tschbg.
Gen. Microcotyle, Gastrocotyle, Axine, Pseudaxine.
4. Fam. Gyrodactylidae v. Ben.
Sub-Fam. 7. Gyrodactylinae Par. et Per.
Gen. Gyrodactylus, Dactylogyrus, Tetraonchus, Diplectanum.
Sub-Fam. 8. Calceostominae Par. et Per.
Gen. Calceostomum, Anoplodiscus.
5. Fam. Aspidobothridae Burm. (= Aspidocotylea Mont.).
Gen. Aspidogaster, Platyaspis, Cotylogaster, Macraspis.
B. Digenea v. Ben. (Malacocotylea Mont.).
6. Fam. Holostomatidae Brandes (= Metastatica Leuckart).
Gen. Diplostomum, Polycotyle, Hemistomum, Holostomum.
7. Fam. Amphistomatidae Mont.
Gen. Amphistomum, Diplodiscus, Gastrodiscus, Homalogaster, Gastrothylax, Aspidocotyle.
8. Fam. Distomatidae Mont.
Gen. Distomum (and sub-genera), Rhopalophorus, Koellikeria, Bilharzia.
9. Fam. Gasterostomatidae Braun.
Gen. Gasterostomum.
10. Fam. Didymozoontidae Mont.
Gen. Didymozoon, Nematobothrium.
11. Fam. Monostomatidae Mont.
Gen. Monostomum, Notocotyle, Ogmogaster, Opisthotrema.