Take a small rod[9] of the clearest and cleanest glass you can procure, free, if possible from blebs, veins, or sandy particles; then by melting it in a lamp with spirit of wine, or the purest and clearest sallad oil, draw it out into exceeding fine and small threads; take a small piece of these threads, and melt the end thereof in the same flame, till you perceive it run into a small drop, or globule, of the desired size; let this globule cool, then fix it upon a thin plate of brass or silver, so that the middle of it may be directly over the center of a very small hole made in this plate, turning it till it is fixed by the before-mentioned thread of glass. When the plate is properly fixed to your microscope, and the object adjusted to the focal distance of the globule, you will perceive the object distinctly and immensely magnified. “By these means,” says Dr. Hooke, “I have been able to distinguish the particles of bodies not only a million times smaller than a visible point, but even to make those visible whereof a million of millions would hardly make up the bulk of the smallest visible grain of sand; so prodigiously do these exceeding small globules enlarge our prospect into the more hidden recesses of nature.”
[9] Lectures and Collections by Dr. Hooke.
Mr. Butterfield, in making of the globules, used a lamp with spirit of wine; but instead of a cotton wick, he used fine silver wire, doubled up and down like a skain of thread.[10] He prepared his glass by beating it to powder, and washing it very clean; he then took a little of this glass upon the sharp point of a silver needle, wetted with spittle, and held it in the flame, turning it about till a glass ball was formed; then taking it from the flame, he afterwards cleaned it with soft leather, and set it in a brass cell.
[10] Philos. Trans. No. 141.
No person has carried the use of these globules so far as Father Di Torre, of Naples, nor been so dexterous in the execution of them; and if others have not been able to follow him in the same line, it may be fairly attributed to a want of that delicacy of touch for adjusting the objects to their focus, and that acuteness of vision which can only be acquired by long practice. Di Torre has also described, more minutely than any other author, the mode of executing these globules, which, as it throws much light upon the preceding description by Dr. Hooke, will not, it is presumed, be unacceptable to the reader.
Three things are necessary for forming of these globules: 1. A lamp and bellows, such as are used by the glass-blowers. 2. A piece of perfect tripoli. 3. A variety of small glass rods. When the flame of the lamp is blown in an horizontal direction, it will be found to consist of two parts; from the base to about two thirds of its length, it is of a white colour; beyond this, it is transparent and colourless. It is this transparent part which is to be used for melting the glass, because by this it will not be in the least sullied; but it will be immediately soiled, if it touch the white part of the flame. The part of the glass which is presented to the flame, ought to be exceeding clean, and great care should be taken that it be not touched by the fingers. If the glass rod has contracted any spots, it must either be thrown away, or the parts that are spotted must be cut off.
The piece of tripoli which is to be used in forming the globules, should be flat on one side, and so large that it may be handled conveniently, and protect the fingers from the flame. A piece four or five inches long, and three or four inches thick, will answer very well. The best tripoli for this purpose is of a white colour, with a fine grain, heavy and compact, and which, after it has been calcined, is of a red colour. This kind resists the fire best, is not apt to break when calcined, and the melted glass does not adhere to it. To calcine this tripoli, cover it well all round with charcoal nearly red hot, leaving it thus till the charcoal is quite cold; it may then be taken out. Let several hemispherical cavities be made on the flat side of the tripoli; they should be of different sizes, nicely polished, and neatly rounded at the edges, in order to facilitate the entrance of the flame. The large globules are to be placed in the large cavities, and the minuter ones, in the small cavities. The holes in the tripoli must never be touched with the finger. If it be necessary to clean them, it should be done with white paper; the larger globules may be cleaned with wash leather. The glass rods should be of various sizes, as of 1⁄10th, 1⁄20th, 1⁄30th of an inch in diameter, as clean and free from specks and bubbles as possible.
TO MAKE SMALL GLASS MICROSCOPIC GLOBULES.
Take two rods of glass, one in each hand, place their extremities close to each other, and in the purest part of the flame; when you perceive the ends to be fused, separate them from each other; the heated glass following each rod, will be finer, in proportion to the length it is drawn to, and the smallness of the rod; in this manner you may procure threads of glass of any degree of fineness. Direct the flame to the middle of the thread, and it will be instantly divided into two parts. When one of the threads is perfectly cool, place it at the extremity of the flame, by which it will be rendered round; and, if the thread of glass be very fine, an exceeding small globule will be formed. This thread may now be broke off from the rod, and a new one may be again drawn out as before, by the assistance of the other glass rod.
The small ball is now to be separated from the thread of glass; this is easily effected by the sharp edge of a piece of flint. The ball should be placed in a groove of paper, and another piece of paper be held over it, to prevent the ball from flying about and being lost. A quantity of globules ought to be prepared in this manner; they are then to be cleaned, and afterwards placed in the cavities of the tripoli, by means of a delicate pair of nippers. The globules are now to be melted a second time, in order to render them completely spherical; for this purpose, bring one of the cavities near the extremity of the flame, directing this towards the tripoli, which must be first heated; the cavity is then to be lowered, so that the flame may touch the glass, which, when it is red hot, will assume a perfect globular form; it must then be removed from the flame, and laid by; when cold, it should be cleaned, by rubbing between two pieces of white paper. Let it now be set in a brass cap, to try whether the figure be perfect. If the object be not well defined, the globule must be thrown away. Though, if it be large, it may be exposed two or three times to the flame. When a large globule is forming, it should be gently agitated by shaking the tripoli, which will prevent its becoming flat on one side. By attending to these directions, the greater part of the globules will be round and fit for use. In damp weather, notwithstanding every precaution, it will often happen, that out of forty globules, four or five only will be fit for use.