Fig. 28.
Puncturing a make-believe cell after it has been lying in water.
Fig. 29.
Same as Fig. 28 after needle is removed.
42. Diffusion through an animal membrane.—For this experiment we may use a thistle tube, across the larger end of which should be stretched and tied tightly a piece of a bladder membrane. A strong sugar solution (three parts sugar to one part water) is now placed in the tube so that the bulb is filled and the liquid extends part way in the neck of the tube. This is immersed in water within a wide-mouth bottle, the neck of the tube being supported in a perforated cork in such a way that the sugar solution in the tube is on a level with the water in the bottle or jar. In a short while the liquid begins to rise in the thistle tube, in the course of several hours having risen several centimeters. The diffusion current is thus stronger through the membrane in the direction of the sugar solution, so that this gains more water than it loses.
We have here two liquids separated by an animal membrane, water on the one hand which diffuses readily through the membrane, while on the other is a solution of sugar which diffuses through the animal membrane with difficulty. The water, therefore, not containing any solvent, according to a general law which has been found to obtain in such cases, diffuses more readily through the membrane into the sugar solution, which thus increases in volume, and also becomes more dilute. The bladder membrane is what is sometimes called a diffusion membrane, since the diffusion currents travel through it.
43. In this experiment then the bulk of the sugar solution is increased, and the liquid rises in the tube by this pressure above the level of the water in the jar outside of the thistle tube. The diffusion of liquids through a membrane is osmosis.
44. Importance of these physical processes in plants.—Now if we recur to our experiment with spirogyra we find that exactly the same processes take place. The protoplasmic membrane is the diffusion membrane, through which the diffusion takes place. The salt solution which is first used to bathe the threads of the plant is a stronger solution than that of the cell-sap within the cell. Water therefore is drawn out of the cell-sap, but the substances in solution in the cell-sap do not readily move out. As the bulk of the cell-sap diminishes the pressure from the outside pushes the protoplasmic membrane away from the wall. Now when we remove the salt solution and bathe the thread with water again, the cell-sap, being a solution of certain substances, diffuses with more difficulty than the water, and the diffusion current is inward, while the protoplasmic membrane moves out against the cell wall, and turgidity again results. Also in the experiments with salt and sugar solutions on the leaves of geranium, on the leaves and stems of the seedlings, on the tissues and cells of the beet and carrot, and on the root hairs of the seedlings, the same processes take place.
These experiments not only teach us that in the protoplasmic membrane, the cell wall, and the cell-sap of plants do we have structures which are capable of performing these physical processes, but they also show that these processes are of the utmost importance to the plant; not only in giving the plant the power to take up solutions of nutriment from the soil, but they serve also other purposes, as we shall see later.