Fig. 109.
Spores of mucor, and different stages of germination.

243. How the gonidia germinate.—We should find at this time many of the gonidia extended on one side into a tube-like process the length of which varies according to time and temperature. The short process thus begun continues to elongate. This elongation of the plant is growth, or, more properly speaking, one of the phenomena of growth.

244. The germ tube branches and forms the mycelium.—In the course of a day or so branches from the tube will appear. This branched form of the threads of the fungus is, as we remember, the mycelium. We can still see the point where growth started from the gonidium. Perhaps by this time several tubes have grown from a single one. The threads of the mycelium near the gonidium, that is, the older portions of them, have increased in diameter as they have elongated, though this increase in diameter is by no means so great as the increase in length. After increasing to a certain extent in diameter, growth in this direction ceases, while apical growth is practically unlimited, being limited only by the supply of nutriment.

245. Growth in length takes place only at the end of the thread.—If there were any branches on the mycelium when the culture was first examined, we can now see that they remain practically the same distance from the gonidium as when they were first formed. That is, the older portions of the mycelium do not elongate. Growth in length of the mycelium is confined to the ends of the threads.

246. Protoplasm increases by assimilation of nutrient substances.—As the plant increases in bulk we note that there is an increase in the protoplasm, for the protoplasm is very easily detected in these cultures of mucor. This increase in the quantity of the protoplasm has come about by the assimilation of the nutrient substance, which the plant has absorbed. The increase in the protoplasm, or the formation of additional plant substance, is another phenomenon of growth quite different from that of elongation, or increase in bulk.

247. Growth of roots.—For the study of the growth of roots we may take any one of many different plants. The seedlings of such plants as peas, beans, corn, squash, pumpkin, etc., serve excellently for this purpose.

248. Roots of the pumpkin.—The seeds, a handful or so, are soaked in water for about 12 hours, and then placed between layers of paper or between the folds of cloth, which must be kept quite moist but not very wet, and should be kept in a warm place. A shallow crockery plate, with the seeds lying on wet filter paper, and covered with additional filter paper, or with a bell jar, answers the purpose well.

The primary or first root (radicle) of the embryo pushes its way out between the seed coats at the small end. When the seeds are well germinated, select several which have the root 4-5 cm long. With a crow-quill pen we may now mark the terminal portion of the root off into very short sections as in [fig. 110]. The first mark should be not more than 1 mm from the tip, and the others not more than 1mm apart. Now place the seedlings down on damp filter paper, and cover with a bell jar so that they will remain moist, and if the season is cold place them in a warm room. At intervals of 8 or 10 hours, if convenient, observe them and note the farther growth of the root.