The work of Mikimoto is not the only attempt now being made in Japan to produce pearls. A letter from Dr. T. Nishikawa, of the Tokio Imperial University, states: “It is a great pleasure for me to tell you that I am studying pearl formation and pearl-oyster culture in the university laboratory, and recently I have got my pearl laboratory at Fukura, on the Island of Awaji, where I began the pearl-culture work this summer (1907). Fortunately, I found the cause of Japanese pearl formation, i.e., the reason why and how the pearl is produced in the tissue of an oyster. I made practical application of this theory with great prospects for producing the natural and true pearls at will.”

Among the most interesting of the pearl-culture enterprises are those of the Compañia Criadora de Concha y Perla, under the direction of Sr. Gaston J. Vives, in the Gulf of California. This company has an extensive station at San Gabriel, near La Paz, where breeding oysters are placed in prepared chests or cages for collecting the spat on trays. After remaining there for several weeks or months, the young mollusks are removed to prepared places (viveros) for further growth. Experiments are now made in depositing them between a series of parallel dams alternately touching each shore of a lagoon, thus developing a current of water over the oysters for conveying food to them, and thus hastening their growth.

In efforts to increase the output of pearls, attention has been given to the possibilities for extending the area and production of the reefs, and for stocking new areas and replenishing exhausted ones, thus bringing the pearl-bearing mollusks to maturity in greater abundance.

Although theoretically it does not seem a very difficult undertaking to cultivate the pearl-oysters by methods somewhat similar to the cultivation of edible oysters and clams, in no part of the world has this been successfully done on an extensive scale. While in certain minor cases, the areas of some species of pearl mollusks have been extended indirectly through man’s agency—as the range of the Red Sea pearl-oyster into the Mediterranean since the Suez Canal was opened—there is no well-known instance in which new areas have been abundantly populated through direct efforts.

In the chapter on the pearl fisheries of Asia are noted the hitherto unsuccessful efforts made in Ceylon and India to preserve the young and immature oysters on the storm-swept reefs by removing them to less exposed areas. This has received close attention from the Ceylon authorities during the last two years. Other practical measures which are recommended for that region include “cultching,” or the deposit of suitable solid material, such as shells or broken stone, to which the young oysters can attach themselves; thinning out overcrowded reefs, and cleaning the beds by means of a dredge, thereby removing starfish and other injurious animals. The attempts made by individuals and associations to extend the range of the reefs on the coast of Australia, among the Tuamotu Islands, in the Gulf of California, and some other localities, are noted in the appropriate chapters. But it may be stated that in most instances lack of adequate police protection has been not the least of the difficulties with which these experiments have had to contend.

Nor has much greater success followed upon efforts to prevent the exhaustion of the reefs and productive grounds through overfishing, except in those instances in which the government exercises a proprietory interest and determines the season, the area to be fished, and the quantity of mollusks to be removed. The most prominent instance of this is in Ceylon, where the fishery has been restricted to such seasons and periods as appeared to insure the maximum yield of pearls. Without restriction upon the fishery, the pearl-oyster in that populous region would doubtless become almost extinct in a few years. Another instance of proprietory interest on the part of the government is in some of the German States, where pearl fishing has been regulated and restricted for centuries. But there the sewage from cities and factories has accomplished almost as effectively, if less rapidly, what unrestricted fishing would have done.

Much attention has been given to the subject of pearl-culture in Bavaria, where the government has granted a small subsidy to encourage this industry, and a model pearl-mussel bank has been established in one of the brooks for the rational culture of the mussels.

On the Australian coast, the only theoretical protection of consequence is the restriction on taking small or immature oysters; but, owing to the great area over which the fisheries are prosecuted there, it has not been possible to enforce the regulations. At some of the Pacific islands and elsewhere, interdictions exist as to use of certain apparatus of capture, but this is intended for the purpose of reserving the industry to dependent natives rather than for protecting the reefs. Several efforts have been made to insure adequate protection for the Unios in our American rivers, but nothing in this direction has yet been accomplished by legislative enactment, except in Illinois.

Reference has already been made to the parasitic stage of Unios.[[337]] The attachment of the newly-hatched mollusks to the gills or fins of a fish is entirely a matter of chance, and unless this takes place they die within a few days. Under natural conditions the fish thus infected will rarely be found carrying as many of the parasitic Unios as they can without serious injury. If the fish are placed in a tank or a pond containing large numbers of newly-hatched Unios, it is possible to bring about the attachment of hundreds of them for every one that would be found there by chance of nature. A fish six inches in length may thus be made to carry several hundred parasitic Unios, and thus a thousand fish artificially infected may do the work of several hundred thousand in a state of nature. Experiments with small numbers of fish under observation in the laboratory indicate that their infection on a large scale is entirely possible, and the experiment by Messrs. Lefevre and Curtis now in progress at La Crosse, Wisconsin, in which over 25,000 young fish have been infected, gives every indication that such work may be begun even with the scanty knowledge now possessed.

Since it has already been shown that the production of pearls is an abnormal condition, it does not follow that an increase in the quantity of mollusks would necessarily result in a corresponding increase in the yield of pearls. Indeed, it might even be that the artificial conditions bringing about an enhanced prosperity and abundance of the mollusks would result in a corresponding decrease in the product of gems, the improved surroundings impairing if not destroying the conditions to which the pearls owe their origin. This has resulted in directing efforts toward abnormally increasing the abundance of pearls in a definite number of mollusks.