There are also small breathing holes known as lenticels scattered through the surface of the bark. These can easily be seen in a young woody stem of apple, beech, or horse-chestnut.
Experiment to show that the skin of the potato (a stem) retards evaporation.
Proof that Food passes down the Stem.—If freshly cut willow twigs are placed in water, roots soon begin to develop from that part of the stem which is under water. If now the stem is girdled by removing the bark in a ring just above where the roots are growing, the latter will eventually die, and new roots will appear above the girdled area. The food material necessary for the outgrowth of roots evidently comes from above, and the passage of food materials takes place in a downward direction just outside the wood in the layer of bark which contains the bast fibers and sieve tubes. This experiment with the willow explains why it is that trees die when girdled so as to cut the sieve tubes of the inner bark. The food supply is cut off from the protoplasm of the cells in the part of the tree below the cut area. Many of the canoe birches of our Adirondack forest are thus killed, girdled by thoughtless visitors. In the same manner mice and other gnawing animals kill fruit trees. Food substances are also conducted to a much less extent in the wood itself, and food passes from the inner bark to the center of the tree by way of the pith plates. This can be proved by testing for starch in the pith plates of young stems. It is found that much starch is stored in this part of the tree trunk.
Experiment to show that food material passes down in the inner bark.
In what Form does Food pass through the Stem?—We have already seen that materials in solution (those substances which will dissolve in the water) will pass from cell to cell by the process of osmosis. This is shown in the experiment illustrated in the figure. Two thistle tubes are partly filled, one with starch and water, the other with sugar and water, and a piece of parchment paper is tied over the end of each. The lower ends of both tubes are placed in a glass dish under water. After twenty-four hours, the water in the dish is tested for starch, and then for sugar. We find that only the sugar, which has been dissolved by the water, can pass through the membrane.
Experiment to show osmosis of sugar (right hand tube) and non-osmosis of starch (left hand tube).
Digestion.—Much of the food made in the leaves is stored in the form of starch. But starch, being insoluble, cannot be passed from cell to cell in a plant. It must be changed to a soluble form, for otherwise it could not pass through the delicate cell membranes. This is accomplished by the process of digestion. We have already seen that starch is changed to grape sugar in the corn by the action of a substance (an enzyme) called diastase. This process of digestion seemingly may take place in all living parts of the plant, although most of it is done in the leaves. In the bodies of all animals, including man, starchy foods are changed in a similar manner, but by other enzymes, into soluble grape sugar.