With new energy, vigor, and hope, Portolá set out again for the search of Monterey, accompanied by Serra as well as Crespí. This time the attempt was successful. They recognized the bay, and on June 3, 1770, a shelter of branches was erected on the beach, a cross made ready near an old oak, the bells were hung and blessed, and the services of founding began. Padre Serra preached with his usual fervor; he exhorted the natives to come and be saved, and put to rout all infernal foes by an abundant sprinkling of holy water. The Mission was dedicated to San Carlos Borromeo.

Thus two of the long desired Missions were established, and the passion of Serra's longings, instead of being assuaged, raged now all the fiercer. It was not long, however, before he found it to be bad policy to have the Missions for the Indian neophytes too near the presidio, or barracks for the soldiers. These latter could not always be controlled, and they early began a course which was utterly demoralizing to both sexes, for the women of a people cannot be debauched without exciting the men to fierce anger, or making them as bad as their women. Hence Serra removed the Missions: that of San Diego six miles up the valley to a point where the ruins now stand, while that of San Carlos he re-established in the Carmelo Valley.

The Mission next to be established should have been San Buenaventura, but events stood in the way; so, on July 14, 1771, Serra (who had been zealously laboring with the heathen near Monterey), with eight soldiers, three sailors, and a few Indians, passed down the Salinas River and established the Mission of San Antonio de Padua. The site was a beautiful one, in an oak-studded glen, near a fair-sized stream. The passionate enthusiasm of Serra can be understood from the fact that after the bells were hung from a tree, he loudly tolled them, crying the while like one possessed: "Come, gentiles, come to the Holy Church, come and receive the faith of Jesus Christ!" Padre Pieras could not help reminding his superior that not an Indian was within sight or hearing, and that it would be more practical to proceed with the ritual. One native, however, did witness the ceremony, and he soon brought a large number of his companions, who became tractable enough to help in erecting the rude church, barracks and houses with which the priests and soldiers were compelled to be content in those early days.

On September 8, Padres Somera and Cambon founded the Mission of San Gabriel Arcángel, originally about six miles from the present site. Here, at first, the natives were inclined to be hostile, a large force under two chieftains appearing, in order to prevent the priests from holding their service. But at the elevation of a painting of the Virgin, the opposition ceased, and the two chieftains threw their necklaces at the feet of the Beautiful Queen. Still, a few wicked men can undo in a short time the work of many good ones. Padre Palou says that outrages by soldiers upon the Indian women precipitated an attack upon the Spaniards, especially upon two, at one of whom the chieftain (whose wife had been outraged by the man) fired an arrow. Stopping it with his shield, the soldier levelled his musket and shot the injured husband dead. Ah! sadness of it! The unbridled passions of men of the new race already foreshadowed the death of the old race, even while the good priests were seeking to elevate and to Christianize them. This attack and consequent disturbance delayed still longer the founding of San Buenaventura.