MICROSCOPICAL EXAMINATION.
Mr. Nicol, who first suggested the method now generally adopted for preparing fossil wood, coal, &c. for microscopical examination, and which was employed by Mr. Witham in the illustrations of his beautiful work on the structure of fossil plants,[43] has so clearly explained the process, that by a little practice the student will be able to prepare specimens sufficiently thin for every useful purpose. Several lapidaries in London, (see list at the end of this work,) polish and mount fossil vegetables and other substances, in a very superior manner; but their charges are high, and they frequently injure specimens by grinding them too thin, and thus obliterating structure. I would recommend that a small chip of the specimen, if possible in a radial direction, should be examined by reflected light, always beginning with the lowest object-glass and eye-piece, and ascending to the highest power; at first without any preparation;[44] subsequently the object should be immersed in oil of turpentine, which will render it somewhat transparent, and it then should be examined by transmitted light. By this exploration we may detect structure, and ascertain if the specimen be worth the trouble or expense of further preparation.
[43] Observations on Fossil Vegetables. 4to. 1833.
[44] The drawings in Plate V. figs. 2 and 3, of fossil coniferous wood, were from chips seen by reflected light, and without any preparation.
Coal may be prepared for examination, by removing with a sharp knife a thin pellicle, or a minute scraping; immerse it in a drop of oil of turpentine on a piece of glass; then add a little Canada balsam, and hold the glass over the flame of a lamp till the balsam is spread evenly over the specimen. But without any preparation, the surface of coal recently broken may be successfully investigated. One of the most interesting examples of coniferous structure in coal that my cabinet contains, was discovered by my son in a piece lying on the fire, which had been cracked by the heat; and I have another fragment, showing the spiral vessels, and coniferous glands, which the Rev. J. B. Reade obtained under similar circumstances. But for choice specimens, the following method is to be employed; and in many cases no other plan will succeed. Sections of teeth, bone, marble, &c. may be prepared by a like process.
MODE OF PREPARING SLICES OF FOSSIL WOOD FOR MICROSCOPICAL EXAMINATION.
"Let a thin slice be cut off from the fossil wood, in a direction perpendicular to the length of its fibres—the slice thus obtained must be ground perfectly flat, and polished. The polished surface is then to be cemented to a piece of plate glass (3 in. long and 1 in. wide) by Canada balsam—a thin layer of balsam must be applied to the polished surface of the slice, and also to one side of the glass—the slice and the glass are now to be laid on any thin plate of metal, and gradually heated over a slow fire, or a spirit lamp, to concentrate the balsam. The heat must not be so great as to throw the balsam into a state of ebullition; for if air bubbles be formed, it is difficult to get rid of them, and if not removed they will prevent the complete adhesion of the two surfaces when applied to each other; the heat of the metal should never be so great that the fingers may not be held in contact with it for a few seconds without inconvenience. When air bubbles are formed, they should be displaced by a small piece of wood tapering to a point; when the balsam is thought to be sufficiently concentrated, and all the air bubbles have disappeared, the slice and glass may be taken from the heated metal, and pressed closely together; a slight degree of pressure will suffice to expel the super-abundant balsam, and this will be facilitated by gently sliding the specimen to and fro on the glass; by this kind of motion any air that may have got entangled when the two surfaces were brought in contact, will also be removed. When the whole is cooled down to the temperature of the air, and the balsam has become solid, that part which adheres to the surface of the glass surrounding the slice should be scraped off with the point of a penknife; and by this operation, it will at once be seen whether the balsam has undergone the requisite concentration; for if it flakes off before the knife, it will be found that the slice and glass will cohere so firmly, that in the subsequent grinding, there will be no risk of their separating from each other; but if the balsam has not been sufficiently concentrated, it will slide before the knife, and in that case the two bodies will not adhere with requisite firmness. If the layer of balsam applied to the two surfaces be not too thick, its due concentration will be accomplished in four or five minutes, provided the application of the heat be properly regulated. The slice must now be ground to that degree of thinness which will permit its structure to be seen by the help of a microscope. This will be accomplished by rubbing the slice, by a rapid circular motion with the hand, on a piece of sheet lead, supplied with a little emery (size No. 1.) moistened with water; when the emery ceases to act, the muddy matter remaining should be removed, and a fresh portion of emery applied; this must be repeated until the surface of the slice is perfectly flat; a sheet of copper must then be substituted for the lead, and the fossil ground as smooth as possible by flower of emery, freed from its coarser parts. The surface may then be polished by friction, with crocus or rotten stone, on a transverse section of any soft wood."[45]
[45] Mr. Witham, Observations on Fossil Vegetables.