Text Book of Biology, Part 1: Vertebrata

by H. G. Wells

For such dissection as the subject-matter of this book requires, the following appliances will be needed:--

(a) Two or three scalpels of various sizes.
(b) Scissors, which must taper gradually, have straight blades, and be pointed at the ends, and which must bite right up to the tips (or they are useless). Two pairs, small and large, are advisable.
(c) Forceps, which must hold firmly, and meet truly at the points.
(d) Two needles set in wooden handles.
(e) An ordinary watchmaker's eye-glass is very helpful, but not indispensable.
(f) A dissecting dish-- an ordinary pie dish will do-- into which melted paraffin wax has been poured, to the depth of, say, three-quarters of an inch, and allowed to solidify. (This wax may be blackened by mixture with lampblack. If the wax floats up at any time, it can, of course, be remelted. Or it may be loaded with lead.)
(g) A rough table or board (for the rabbit and dog-fish).
(h) Blanket pins, and ordinary pins.
(i) A pickle or other wide-mouthed jar, and some common, methylated spirit.
(j) A microscope, with low power of 1 inch or 1/2 inch, and high power 1/6 inch or 1/4 inch. Glass slips and cover glasses, and a bottle of very weak (1 per cent.) solution of salt.

Animals for dissection may be obtained from the recognised dealers, who usually advertise in such scientific periodicals as Nature, Natural Science, and Knowledge. Sinel (naturalist, Jersey) is the most satisfactory dealer in dog-fish in our experience; Bolton (Malvern) will supply Amphioxus through the post; frogs and rabbits may be obtained anywhere. The tame variety of rabbit is quite satisfactory for the purpose of dissection.

The following notes may possibly be of some use to the student; they follow the lines of work arranged by the author for the evening classes of the University Tutorial College, classes considerably restricted as regards time, when compared with ordinary laboratory workers. Most of the sections below occupied about three hours, but for a student working alone they are more likely to take four or five, and even then it is not probable that they will be so satisfactory as if performed under skilled supervision. There are many points extremely difficult to convey verbally which are elucidated at once by actual demonstration upon a specimen. Each of these dissections should be repeated, and it is well if a different condition of the type is selected for the repetition-- an old one if the first specimen was immature, a female if the first was a male.

-The Rabbit_

May be killed by chloroform, or potassium cyanide, or drowned. It may also be readily suffocated with house-hold gas. It should be killed immediately before use, as otherwise the gastric juice attacks the wall of the stomach, and the dissection is, in consequence, rendered extremely disagreeable. A very young rabbit is unsatisfactory as regards the genitalia, but otherwise there is no objection to a little one, and it has this advantage-- that it may be immersed more conveniently under water, in a large pie dish, for purposes of fine dissection. The external features of the animal should be examined: eyelids, whiskers and teeth, toes, anus, perineal space on either side of the same, urogenital opening, and position of the ribs, vertebral column, and limb girdles beneath the skin should be made out. Then the animal should be pinned out through the legs, the ventral surface uppermost, the skin opened up along the middle line from pelvic girdle to symphyses of jaw; separated from the body wall below by means of the handle of a scalpel, and turned back; and then the abdominal wall should be cut into and two flaps pinned back to expose its contents. Note the xiphisternum. The caecum and colon will be recognised ([Section 16]); the stomach, the right and left central, and left lateral lobes of the liver will probably be apparent; and the urinary bladder (especially if distended) in the middle line behind. Without any further dissection, but simply by turning the parts over, all the structures of the abdomen in Figure 1, [Sheet 1], will be identified. Seek especially for and note particularly, the gall bladder, bile duct, and portal vein, pancreatic duct, sacculus rotundus, vermiform appendix, ureters (by pulling urinary bladder forward), genital ducts (looping over ureters), spleen, kidneys, and adrenals. The vena cava inferior is seen dorsally. The genital duct guides the student to the genital gland; if the subject is a male, the testes may be exposed by dissection, or by pulling the vas deferens gently the scrotal sac will be turned inside out, and the testes brought into view. The ovary lies exposed without dissection posterior to the kidney. Examine all this carefully, and make small sketches of points of interest-- the duodenal loop and the pyloric end of the stomach, for instance; the meeting of colon, caecum, and sacculus rotundus again; or the urinary bladder and adjacent parts. Note the dorsal aorta and vena cava and their connexions behind. (Compare figure of circulation.) Cut through pelvic girdle, and remove one hind leg, to see bladder and genital ducts better (compare [Sheet 10]). Wash away any blood that may flow. Turn all the intestines over to the animal's right, and see the dorsal aorta and vena cava inferior of the abdomen, the inferior mesenteric artery, and the spermatic (or ovarian) artery (compare, of course, with figure in book). In front, immediately dorsal to the spleen, is a variable quantity of lymphoidal tissue, which must be very carefully cleared to see the superior mesenteric and coeliac arteries. Separate Spigelian lobe from stomach, and look for vagus nerve descending by oesophagus, solar plexus around the superior mesenteric artery, and thrown up very distinctly by the purple vena cava inferior beneath, and the splanchnic nerve. To see the abdominal sympathetic behind, gently remove the peritoneum that lies on either side of the aorta; blood-vessels will be seen running in between the vertebral bodies, and the sympathetic chain, with its ganglia, made out very distinctly, as it runs across them longitudinally. Now cut oesophagus just in front of stomach, and cut the rectum, cut through the mesentery supporting the intestine, and remove and unravel alimentary canal; cut open, wash out, and examine caecum and stomach. Bleeding to a considerable extent is inevitable, chiefly from the portal vein. The liver had better remain if the same rabbit is to serve for the second dissection.

Second Dissection.-- Skin front of thorax and neck. Note subclavian veins running out to fore limbs-- avoid cutting these. Cut through ribs and remove front of thorax, to expose its contents; cut up middle line of neck, and clear off small muscle bands, to expose bloodvessels; pick away carefully whatever is left of thymus gland; make out structure of heart and blood-vessels, as described, in Chapter 3; note larynx and trachea. Now proceed to the examination of the nerves of this region. See phrenic nerve, by vena cava inferior, and between heart and lungs, and sympathetic, running over the heads of the ribs. By the common carotids will be found the large white vagus nerve, the greyish sympathetic, and a small branch of X., the depressor. Make out branches of X. named in text. The big white cervical spinal nerves will be evident dorsally. Clear forward into the angle between the jaw and the bulla tympani, to see XII. and XI.; IX. will be found, lying deeper, dorsal to the carotid artery and body of the hyoid. Compare with figure given of this. Skin the cheek, and see VII. running over it. Cut through malar and remove it; cut through lower jaw-bone and turn it back, to see the third branch of the fifth nerve on its inner side; examine the muscles of eyeball, and remove it, to expose the first and second branches of V.-- the latter is especially deep within orbit. Remove, open, wash out, and examine the heart. Shave off the dorsal wall of cranium, to expose hemispheres of brain, and then put the head in strong spirit for a week or so. With a second rabbit, this dissection may advantageously be varied by removing the lower jaw, cutting -up- [through] soft palate, and observing openings of the Eustachian tubes. [The tonsils (on the ventral side of the soft palate) must not confused with these.] The heart should also be cut out, washed out and examined (Compare Sections [38], [44].)

Third Dissection.-- (Before this is performed the mammalian skull should have been studied and examined.) Take the head of a rabbit, the brain of which has been hardened by spirit, and carefully remove cranium; be particularly careful in picking away the periotic bone, on account of the flocculi of cerebellum. It is difficult to avoid injury to the pituitary body embedded in the basisphenoid bone. Examine with the help of [Sheet 8]. Make the sections there indicated.

-The Frog_

May be killed by drowning in dilute methylated spirit, or by chloroform. Take a recently-killed frog, and examine a drop of its blood, spread out on a glass slip, under the microscope; compare it with your own. Before using the high power, put a cover glass over the object, of course. Scrape the roof of the mouth of the frog gently, to obtain ciliated epithelium; and mount in very weak salt solution-- the cilia will still be active. Squamous epithelium may be seen by the student similarly scraping the interior of his own cheek. Take a piece of muscle from one of the frog's limbs, tease out with needles upon a glass slip, and examine. To see the striations clearly, the high power will be needed. Compare a piece of muscle from the wall of the alimentary canal. Similarly examine nerve and connective tissue.