Outlines of dairy bacteriology, 10th edition / A concise manual for the use of students in dairying

by H. L. Russell; E. G. Hastings

CHAPTER V.

FERMENTATIONS OF MILK.

Milk, under normal conditions, is always contaminated with bacteria coming from the most varied sources. If it is produced under clean conditions, the number of bacteria will be small, but in any case, the number of kinds of bacteria that find their way into milk will be large. Many of them find in milk at ordinary temperatures suitable conditions for growth; they use a portion of some of the constituents of the milk as food, producing certain other compounds that are known as "by-products." These by-products impart to milk a taste and odor that is not found in fresh milk. The effect of the action of bacteria may also be made evident by the change in the appearance of the milk. When these various changes become evident to the senses, either by taste, smell or sight, the milk usually is so modified as to be unfit for many ordinary purposes. The preservation of milk, a subject to be treated later, is a study of the ways of preventing or retarding the growth of bacteria in milk, and thus delaying the time when evidences of their action first become apparent.

Each class of bacteria produces more or less specific changes in the milk as a result of their growth. Certain bacteria are of the greatest benefit to the butter and cheese maker, while others are distinctly harmful to the manufacturer of dairy products. The changes produced by the different bacteria are called "fermentations" of milk, each being most commonly named from the most important by-product formed.

Acid fermentation of milk. Fresh milk has a sweet taste and little or no odor, but if it is allowed to stand at ordinary temperatures, it sours; the taste is no longer sweet because the sweetness of the sugar of the milk is masked by the acid produced from the decomposition of a portion of the sugar by the bacteria. The change in odor and taste of milk is apparent long before the appearance is altered and increases in intensity as the acid-fermentation progresses. The first alteration in appearance is most usually one of consistency; the liquid milk is transformed into a semi-solid mass. The terms "curdling" and "sour" are usually synonymous. Milk is, however, often said to be sour as soon as the acid fermentation has progressed to a point where it is evident to taste or smell. This process of souring, or the acid fermentation is so common a change that raw milk which does not show this type of fermentation is looked upon with suspicion, and, usually, justly so. The process in the past was thought to be something inherent in the milk, a natural and inevitable change. It is now known that this is not so, but that it is due to certain kinds of bacteria, and that if these are prevented from getting into milk, it will not sour, but will undergo some other less desirable type of decomposition.

The acid-forming bacteria comprise but a very small part of the total number of organisms that find their way into the milk during its production on the farm, yet in sour milk scarcely any other kinds of bacteria can be found. At ordinary air temperatures, the acid-forming bacteria grow more rapidly in milk than do any other forms, and the acid produced by them renders the milk an unfavorable medium for the growth of other bacteria. This is the reason why milk practically always undergoes the acid fermentation, although it is contaminated with a host of other kinds of bacteria. If a mixture of seeds is sown on low wet ground, certain kinds will grow best; if the same mixture is sown on drier land, other types will find most favorable conditions for growth, and the plants which appeared on the low land will not appear. The same condition is found in milk where the environment is most favorable for the acid-forming bacteria.

Amount of acid formed in milk. In this country the acidity of milk is expressed as so many per cent of lactic acid. A milk that shows an acidity of one per cent should, theoretically, contain one pound of lactic acid in each one hundred pounds of milk. The acid determined does not actually represent lactic acid, as there are other substances in milk which act as acids, with the reagents used in the present methods of determining the acidity of milk. For instance, perfectly fresh milk has an apparent acidity of 0.13 to 0.18 per cent, although no fermentation has occurred. Other acids than lactic are formed in the acid fermentation, but the entire acid content is referred to as lactic when speaking of the acidity of milk. When the developing acidity of milk reaches 0.25 to 0.3 per cent, a sour taste becomes evident and the milk will curdle on heating. When the acidity increases to 0.6 to 0.7 per cent, the milk curdles at ordinary temperatures. The acidity continues, however, to increase until it reaches about 1 per cent, which is the maximum amount that will be produced in milk by the ordinary acid-forming bacteria. Milk contains about 4 per cent of milk sugar, all of which is fermentable. If this were all decomposed by bacteria, the acidity of the milk would actually exceed 4 per cent. It is thus evident that the reason why more acid is not formed in milk is not because of any lack of sugar. The bacteria, like all other kinds of living things, are injured by their own by-products, unless these are constantly removed in some way; in milk the bacteria cannot escape the action of the acid which they themselves have formed, consequently growth ceases. The amount of acid formed is dependent on the kind of bacteria present and on the composition of the milk. Certain bacteria will not produce enough acid to cause the curdling of the milk; still others will form 2 or even 3 per cent. These types, however, do not play any important part in the spontaneous souring of milk.

In milk the acid first formed combines with the ash constituents and the casein to form salts which do not seriously affect the growth of the bacteria. Ultimately, the limit of the ash and casein to take up acid is reached, and free lactic acid which is harmful to bacterial growth appears. If the content of casein and ash constituents is high, a higher degree of acidity will be reached than in a milk with a lower content. If a large part of the volume of the milk is made up of a compound that has no role whatever in the acid fermentation, such as the butter fat in cream, the amount of acid formed per unit volume of milk will be reduced, since in determining the acidity, a definite volume of milk is taken, and the acidity is expressed, as such a per cent of this amount.